Hierarchical mechanism of amino acid sensing by the T-box riboswitch
Nature Communications, 2018 Riboswitches on 5′ ends of mRNAs are important for bacterial gene regulation. Here the authors probe the mechanism of a tRNA aminoacylation sensing T-box riboswitch using single-molecule fluorescence microscopy to characterize dynamic solution ...
Krishna C. Suddala +5 more
doaj +1 more source
Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. [PDF]
, 2019 Characterization of single cell metabolism is imperative for understanding subcellular functional and biochemical changes associated with healthy tissue development and the progression of numerous diseases.
Aghaamoo, Mohammad +4 more
core +1 more source
scDA: Single cell discriminant analysis for single-cell RNA sequencing data
Computational and Structural Biotechnology Journal, 2021 Single-cell RNA-sequencing (scRNA-seq) techniques provide unprecedented opportunities to investigate phenotypic and molecular heterogeneity in complex biological systems.
Qianqian Shi +4 more
doaj +1 more source
Microfluidic Single-Cell Manipulation and Analysis: Methods and Applications
Micromachines, 2019 In a forest of a hundred thousand trees, no two leaves are alike. Similarly, no two cells in a genetically identical group are the same. This heterogeneity at the single-cell level has been recognized to be vital for the correct interpretation of ...
Tao Luo, Lei Fan, Rong Zhu, Dong Sun
doaj +1 more source
Molecular Plant-Microbe Interactions, 2004 Resistance and susceptibility in barley to the powdery mildew fungus (Blumeria graminis f. sp. hordei) is determined at the single-cell level. Even in genetically compatible interactions, attacked plant epidermal cells defend themselves against attempted
Torben Gjetting +3 more
doaj +1 more source
SCALA: A complete solution for multimodal analysis of single-cell Next Generation Sequencing data
Computational and Structural Biotechnology Journal, 2023 Analysis and interpretation of high-throughput transcriptional and chromatin accessibility data at single-cell (sc) resolution are still open challenges in the biomedical field.
Christos Tzaferis +5 more
doaj +1 more source
STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods [PDF]
, 2017 The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis.
Cornelis, Senne +5 more
core +2 more sources
Single Cell Analysis in Vascular Biology
Frontiers in Cardiovascular Medicine, 2020 The ability to quantify DNA, RNA, and protein variations at the single cell level has revolutionized our understanding of cellular heterogeneity within tissues.
Nicholas W. Chavkin +2 more
doaj +1 more source
Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis
Molecules, 2016 This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets;
Na Wen +6 more
doaj +1 more source
Deep generative modeling for single-cell transcriptomics. [PDF]
, 2018 Single-cell transcriptome measurements can reveal unexplored biological diversity, but they suffer from technical noise and bias that must be modeled to account for the resulting uncertainty in downstream analyses.
A Regev +39 more
core +1 more source