Results 31 to 40 of about 9,923,505 (317)

Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. [PDF]

, 2019
Characterization of single cell metabolism is imperative for understanding subcellular functional and biochemical changes associated with healthy tissue development and the progression of numerous diseases.
Aghaamoo, Mohammad, Digman, Michelle A, Kamalakshakurup, Gopakumar, Lee, Abraham P, Ma, Ning   +4 more
core   +1 more source

SCENIC: Single-cell regulatory network inference and clustering

Nature Methods, 2017
We present SCENIC, a computational method for simultaneous gene regulatory network reconstruction and cell-state identification from single-cell RNA-seq data (http://scenic.aertslab.org).
S. Aibar, C. González-Blas, Thomas Moerman, V. A. Huynh-Thu, Hana Imrichová, Gert J. Hulselmans, F. Rambow, J. Marine, P. Geurts, J. Aerts, Joost van den Oord, Z. Atak, J. Wouters, S. Aerts   +13 more
semanticscholar   +1 more source

Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection

Frontiers in Medicine, 2020
It has been known that, the novel coronavirus, 2019-nCoV, which is considered similar to SARS-CoV, invades human cells via the receptor angiotensin converting enzyme II (ACE2).
Xin Zou, Ke Chen, Jiawei Zou, Pei-jun Han, Jie Hao, Ze-Guang Han   +5 more
semanticscholar   +1 more source

High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis

Nature Communications, 2023
Single-cell and spatial technologies that profile gene expression across a whole tissue are revolutionizing the resolution of molecular states in clinical samples. Current commercially available technologies provide whole transcriptome single-cell, whole
Amanda Janesick, Robert Shelansky, Andrew D. Gottscho, Florian Wagner, Stephen R. Williams, Morgane Rouault, Ghezal Beliakoff, Carolyn A. Morrison, Michelli F Oliveira, Jordan Sicherman, Andrew Kohlway, Jawad Abousoud, T. Drennon, Seayar Mohabbat, Sarah E. B. Taylor   +14 more
semanticscholar   +1 more source

Microfluidic Single-Cell Manipulation and Analysis: Methods and Applications

Micromachines, 2019
In a forest of a hundred thousand trees, no two leaves are alike. Similarly, no two cells in a genetically identical group are the same. This heterogeneity at the single-cell level has been recognized to be vital for the correct interpretation of ...
Tao Luo, Lei Fan, Rong Zhu, Dong Sun
doaj   +1 more source

scDA: Single cell discriminant analysis for single-cell RNA sequencing data

Computational and Structural Biotechnology Journal, 2021
Single-cell RNA-sequencing (scRNA-seq) techniques provide unprecedented opportunities to investigate phenotypic and molecular heterogeneity in complex biological systems.
Qianqian Shi, Xinxing Li, Qirui Peng, Chuanchao Zhang, Luonan Chen   +4 more
doaj   +1 more source

Dimensionality reduction for visualizing single-cell data using UMAP

Nature Biotechnology, 2018
Advances in single-cell technologies have enabled high-resolution dissection of tissue composition. Several tools for dimensionality reduction are available to analyze the large number of parameters generated in single-cell studies. Recently, a nonlinear
E. Becht, Leland McInnes, John Healy, C. Dutertre, I. Kwok, L. Ng, F. Ginhoux, E. Newell   +7 more
semanticscholar   +1 more source

Differential Gene Expression in Individual Papilla-Resistant and Powdery Mildew-Infected Barley Epidermal Cells

Molecular Plant-Microbe Interactions, 2004
Resistance and susceptibility in barley to the powdery mildew fungus (Blumeria graminis f. sp. hordei) is determined at the single-cell level. Even in genetically compatible interactions, attacked plant epidermal cells defend themselves against attempted
Torben Gjetting, Timothy L. W. Carver, Leif Skøt, Michael F. Lyngkjær   +3 more
doaj   +1 more source

Cross-species single-cell analysis of pancreatic ductal adenocarcinoma reveals antigen-presenting cancer-associated fibroblasts.

Cancer Discovery, 2019
Cancer-associated fibroblasts (CAFs) are major players in the progression and drug resistance of pancreatic ductal adenocarcinoma (PDAC). CAFs constitute a diverse cell population consisting of several recently described subtypes, although the extent of ...
Ela Elyada, M. Bolisetty, Pasquale Laise, W. Flynn, Elise T. Courtois, R. Burkhart, J. Teinor, P. Belleau, Giulia Biffi, M. Lucito, S. Sivajothi, T. Armstrong, Dannielle D. Engle, Kenneth H. Yu, Yuan Hao, elliot k fishman, Youngkyu Park, Jonathan Preall, E. Jaffee, A. Califano, P. Robson, D. Tuveson   +21 more
semanticscholar   +1 more source

Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis.

Cell, 2021
T cells are critical effectors of cancer immunotherapies, but little is known about their gene expression programs in diffuse gliomas. Here, we leverage single-cell RNA sequencing (RNA-seq) to chart the gene expression and clonal landscape of tumor ...
N. Mathewson, O. Ashenberg, I. Tirosh, S. Gritsch, Elizabeth M Perez, Sascha Marx, Livnat Jerby-Arnon, Rony Chanoch-Myers, T. Hara, Alyssa R. Richman, Yoshinaga Ito, Jason W Pyrdol, M. Friedrich, Kathrin Schumann, Michael J. Poitras, P. Gokhale, L. G. Gonzalez Castro, M. Shore, Christine Hebert, Brian Shaw, Heather L. Cahill, M. Drummond, Wubing Zhang, Olamide Olawoyin, H. Wakimoto, O. Rozenblatt-Rosen, P. Brastianos, X. Liu, P. Jones, D. Cahill, M. Frosch, David N. Louis, G. Freeman, K. Ligon, A. Marson, E. Chiocca, D. Reardon, A. Regev, M. Suvà, K. Wucherpfennig   +39 more
semanticscholar   +1 more source