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Single‐Cell RNA Sequencing in Hematological Diseases
PROTEOMICS, 2020Abstract Hematological diseases, including leukemia, lymphoma, and multiple myeloma, are characterized by high heterogeneity with diverse cellular subpopulations. Single‐cell RNA sequencing (scRNA‐seq), a transformational technology, provides deep insights into cell‐to‐cell variation in tumor and microenvironment, allows high ...
Yue Zhu +4 more
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Single-Cell RNA Sequencing with Drop-Seq
2019Drop-Seq is a low-cost, high-throughput platform to profile thousands of cells by encapsualting them into individual droplets. Uniquely barcoded mRNA capture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized
Josephine, Bageritz, Gianmarco, Raddi
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Depletion of Ribosomal RNA Sequences from Single‐Cell RNA‐Sequencing Library
Current Protocols in Molecular Biology, 2016AbstractRecent advances in single‐cell RNA sequencing technologies have revealed high heterogeneity of gene expression profiles in individual cells. However, most current single‐cell RNA‐seq methods use oligo‐dT priming in the reverse transcription steps and detect only polyA‐positive for more accuracy, since there are also polyA‐positive non‐coding ...
Nan, Fang, Rumeysa, Akinci-Tolun
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High-throughput single-cell RNA sequencing
Trends in Plant Science, 2022Tom Denyer, Marja C.P. Timmermans
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Preparation of single cells from tumors for single-cell RNA sequencing
2020Intratumoral heterogeneity of cancer cells and tumor-infiltrating immune cells is increasingly being viewed as a key factor driving tumor progression and response to therapy. Over the past several years, technological advances have created powerful tools to analyze the tumor microenvironment on a single-cell level, including mass cytometry and single ...
Yonina, Bykov +2 more
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Single-Cell RNA Sequencing of Glioblastoma Cells
2018Single-cell RNA sequencing (sc-RNASeq) is a recently developed technique used to evaluate the transcriptome of individual cells. As opposed to conventional RNASeq in which entire populations are sequenced in bulk, sc-RNASeq can be beneficial when trying to better understand gene expression patterns in markedly heterogeneous populations of cells or when
Rajeev, Sen +5 more
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Single-cell RNA-Sequencing in Neuroscience
Neuroforum, 2019Summary Technical innovations in the last decade have allowed to sequence transcriptomes of single cells. Single-cell RNA-sequencing (scRNA-seq) has since then opened the window to a deeper understanding of cellular identity and is becoming a widely used method in molecular biology.
Simone Mayer +3 more
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Single Cell Micro RNA Sequencing Library Preparation
Micro RNAs represent important post-transcriptional regulators in health and are involved in the onset of many diseases. Therefore, the further characterization of physiological miRNA functions is an important basic research question, and miRNAs even have high potential as biomarkers both for prognosis and diagnosis. In order to exploit this potential,Sarah M, Hücker, Stefan, Kirsch
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Stories in Single Cell RNA Sequencing
2022This thesis describes the projects I have worked on since starting the Caltech bioengineering program in fall 2017. The general theme of my projects is that they are all about single cell RNA sequencing (scRNA-seq), spanning the experimental and computational realms.
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