Results 31 to 40 of about 642,878 (258)

Experimental design for single-cell RNA sequencing [PDF]

Briefings in Functional Genomics, 2017
Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet ...
Baran-Gale, Jeanette, Chandra, Tamir, Kirschner, Kristina   +2 more
openaire   +4 more sources

Sample Multiplexing for Retinal Single-Cell RNA-Sequencing [PDF]

iScience
SummaryRare cell populations can be challenging to characterize using microfluidic single-cell RNA sequencing (scRNA-seq) platforms. Typically, the population of interest must be enriched and pooled from multiple biological specimens for efficient collection.
Justin Ma, Ting Kuan Chu, Maria Polo Prieto, Yong Park, Yumei Li, Rui Chen, Graeme Mardon, Benjamin J Frankfort, Nicholas M Tran   +8 more
openaire   +4 more sources

scAnalyzeR: A Comprehensive Software Package With Graphical User Interface for Single-Cell RNA Sequencing Analysis and its Application on Liver Cancer

Technology in Cancer Research & Treatment, 2022
Introduction: The application of single-cell RNA sequencing to delineate tissue heterogeneity and complexity has become increasingly popular. Given its tremendous resolution and high-dimensional capacity for in-depth investigation, single-cell RNA ...
GS Chuwdhury MPhil, Irene Oi-Lin Ng MD, PhD, Daniel Wai-Hung Ho PhD   +2 more
doaj   +1 more source

Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

Computational and Structural Biotechnology Journal, 2023
Obesity affects the function of multiple organs/tissues including the exocrine organ salivary glands. However, the effects of obesity on transcriptomes and cell compositions in the salivary glands have yet been studied by bulk RNA-sequencing and single ...
Heping Huang, Ce Gao, Shuai Wang, Fen Wu, Jinsong Wei, Jinrong Peng   +5 more
doaj   +1 more source

Revolutionizing immunology with single-cell RNA sequencing [PDF]

Cellular & Molecular Immunology, 2019
The immune system is composed of a complex hierarchy of cell types that protect the organism against disease and maintain homeostasis. Identifying heterogeneity of immune cells is the key to understanding the immune system. Advanced single-cell RNA sequencing (scRNA-seq) technologies are revolutionizing our ability to study immunology.
Haide, Chen, Fang, Ye, Guoji, Guo
openaire   +2 more sources

Continual learning approaches for single cell RNA sequencing data

Scientific Reports, 2023
Single-cell RNA sequencing data is among the most interesting and impactful data of today and the sizes of the available datasets are increasing drastically.
Gorkem Saygili, Busra OzgodeYigin
doaj   +1 more source

Comparative Analysis of Single-Cell RNA Sequencing Methods [PDF]

Molecular Cell, 2017
Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and medical questions. However, systematic comparisons of the performance of diverse scRNA-seq protocols are lacking. We generated data from 583 mouse embryonic stem cells to evaluate six prominent scRNA-seq methods: CEL-seq2, Drop-seq, MARS-seq, SCRB-seq, Smart-seq ...
Ziegenhain, Christoph, Vieth, Beate I.H., Parekh, Swati, Reinius, Björn, Guillaumet-Adkins, Amy, Smets, Martha, Leonhardt, Heinrich, Heyn, Holger, Hellmann, Ines, Enard, Wolfgang   +9 more
openaire   +3 more sources

Single-Cell RNA-Sequencing: Assessment of Differential Expression Analysis Methods

Frontiers in Genetics, 2017
The sequencing of the transcriptomes of single-cells, or single-cell RNA-sequencing, has now become the dominant technology for the identification of novel cell types and for the study of stochastic gene expression.
Alessandra Dal Molin, Giacomo Baruzzo, Barbara Di Camillo   +2 more
doaj   +1 more source

Power Analysis of Single Cell RNA-Sequencing Experiments [PDF]

Nature Methods, 2016
AbstractHigh-throughput single cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, and has revealed new cell types, and new insights into developmental process and stochasticity in gene expression.
Svensson, Valentine, Natarajan, Kedar Nath, Ly, Lam-Ha, Miragaia, Ricardo J, Labalette, Charlotte, Macaulay, Iain C, Cvejic, Ana, Teichmann, Sarah A   +7 more
openaire   +4 more sources

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source