Results 31 to 40 of about 980,999 (298)

The Application of Single-Cell RNA Sequencing in Vaccinology

Journal of Immunology Research, 2020
Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher
Andrés Noé, Tamsin N. Cargill, Carolyn M. Nielsen, Andrew J. C. Russell, Eleanor Barnes   +4 more
doaj   +1 more source

Microglia Heterogeneity in Alzheimer’s Disease: Insights From Single-Cell Technologies

Frontiers in Synaptic Neuroscience, 2021
Microglia are resident immune cells in the central nervous system and play critical roles in brain immunity, development, and homeostasis. The pathology of Alzheimer’s disease (AD) triggers activation of microglia.
Hansen Wang
doaj   +1 more source

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing. [PDF]

, 2020
Although heterogeneity is recognized within the murine satellite cell pool, a comprehensive understanding of distinct subpopulations and their functional relevance in human satellite cells is lacking.
Aguilar, Aizarani, Alexander, Alonso-Martin, Arpino, Arpke, Baker, Biernacka, Bigot, Boscher, Brooks, Butler, Chakkalakal, Chakkalakal, Charville, Cheung, Cho, Collins, Dell'Orso, Deng, Der Vartanian, Dumont, Elgort, Fan, Flint, Fukada, Garcia, Garcia, Gayraud-Morel, Gnocchi, Guruharsha, Gvaramia, Hai, Hurley, Inoue, Jiang, Kann, Karantza, Kuang, Kumar, Lee, Liu, Liu, Liu, Machado, Macosko, Mallaney, Marchand, Marti, Martinez, Mauro, Min, Moniot, Montarras, Mourkioti, Ng, Nicol, Ono, Pal, Pala, Pallafacchina, Pan, Pomerantz, Porpiglia, Qing, Qiu, Qiu, R Development Core Team, Rozkalne, Rubenstein, Ryall, Sacco, Satija, Scaramozza, Schiaffino, Schmidt, Schroder, Shea, Sheikh, Shen, Sherwood, Si, Siegel, Simeckova, Smith, Song, Sousa-Victor, Stark, Takahashi, Tierney, Trapnell, Tzioras, Uezumi, van Velthoven, Volonte, Wagner, Weinstein, Wu, Wu, Xu, Yang, Yeh, Yi, Yu, Yu, Zhang, Zheng   +106 more
core   +1 more source

Single-cell RNA sequencing v2

, 2021
Single-cell RNA sequencing (scRNA-seq) allows for transcriptional profiling of individual cells within a heterogenous sample. This protocol describes a method for performing scRNA-seq using handpicked pancreatic islets from organ donors.
Klaus H. Kaestner Lab, Suzanne Shapira
openaire   +1 more source

Pumping the brakes on RNA velocity by understanding and interpreting RNA velocity estimates

Genome Biology, 2023
Background RNA velocity analysis of single cells offers the potential to predict temporal dynamics from gene expression. In many systems, RNA velocity has been observed to produce a vector field that qualitatively reflects known features of the system ...
Shijie C. Zheng, Genevieve Stein-O’Brien, Leandros Boukas, Loyal A. Goff, Kasper D. Hansen   +4 more
doaj   +1 more source

SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology [PDF]

, 2018
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Abdelmoez Mahmoud N., Iida Kei, Kotera Hidetoshi, Nishikii Hidekazu, Oguchi Yusuke, Santiago Juan G., Shintaku Hirofumi, Uemura Sotaro, Yokokawa Ryuji, 錦井 秀和   +9 more
core   +2 more sources

Single cell RNA sequencing research in maternal fetal interface

Frontiers in Cell and Developmental Biology, 2023
The maternal-fetal interface is an essential environment for embryonic growth and development, and a successful pregnancy depends on the dynamic balance of the microenvironment at the maternal-fetal interface.
Qian Chen, Qian Chen, Dan Shan, Dan Shan, Yupei Xie, Yupei Xie, Xingrong Luo, Xingrong Luo, Yuxia Wu, Yuxia Wu, Qiuhe Chen, Qiuhe Chen, Ruihong Dong, Ruihong Dong, Yayi Hu, Yayi Hu, Yayi Hu   +16 more
doaj   +1 more source

Intracerebral Hemorrhage and Ischemic Stroke of Different Etiologies Have Distinct Alternatively Spliced mRNA Profiles in the Blood: a Pilot RNA-seq Study. [PDF]

, 2015
Whole transcriptome studies have used 3'-biased expression microarrays to study genes regulated in the blood of stroke patients. However, alternatively spliced messenger RNA isoforms have not been investigated for ischemic stroke or intracerebral ...
Ander, Bradley P, Dykstra-Aiello, Cheryl, Ho, Carolyn, Hull, Heather, Jickling, Glen C, Liu, DaZhi, Orantia, Miles, Stamova, Boryana, Zhan, Xinhua   +8 more
core   +2 more sources

Experimental design for single-cell RNA sequencing [PDF]

Briefings in Functional Genomics, 2017
Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet ...
Baran-Gale, Jeanette, Chandra, Tamir, Kirschner, Kristina   +2 more
openaire   +4 more sources

Linked optical and gene expression profiling of single cells at high-throughput. [PDF]

, 2020
Single-cell RNA sequencing has emerged as a powerful tool for characterizing cells, but not all phenotypes of interest can be observed through changes in gene expression.
Abate, Adam R, Chang, Kai-Chun, Gartner, Zev J, Liu, Leqian, Siltanen, Christian A, Zhang, Jesse Q   +5 more
core   +1 more source