Results 181 to 190 of about 479,986 (210)
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A Force‐Spectroscopy‐Based Single‐Molecule Metal‐Binding Assay
ChemPhysChem, 2009AbstractQuantifying metal‐binding by force: A quantitative single‐molecule force‐spectroscopy‐based assay is developed to measure the binding affinity of metal ions to proteins. The method uses the unfolding force of a protein as a direct probe to distinguish the apo and metal‐ion‐bound forms of that protein and quantify the partitioning between the ...
Yi, Cao +3 more
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Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins
2011The direct observation of single kinesins and microtubule-associated proteins (MAPs) has become a core tool for cytoskeleton research. We outline several variations to the core experiment that allow the researcher to explore structural and biophysical mechanisms underlying kinesin motility and MAP function.
Susanne, Bechstedt +3 more
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New assay for multiple single molecule enzyme kinetics
SPIE Proceedings, 2005A population of identical proteins has the same amino acid sequence, but there may be subtle differences in local folding that lead to variations in activity. Single molecule studies allow us to understand these subtle differences. Single molecule experiments are usually time consuming and difficult because only a few molecules are observed in one ...
Alan I. Lee, James P. Brody
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An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Journal of Visualized Experiments, 2022Phosphorylation is a necessary posttranslational modification that regulates protein function and directs cell signaling outcomes. Current methods to measure protein phosphorylation cannot preserve the heterogeneity in phosphorylation across individual proteins.
Elizabeth M, Bailey +7 more
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Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding
2019Cotranscriptional RNA folding plays important roles in gene regulation steps such as splicing, transcription termination, and translation initiation. Progression of our understanding of cotranscriptional RNA folding mechanisms is still retarded by the lacking of experimental tools to study the kinetics of cotranscriptional RNA folding properly. In this
Heesoo, Uhm, Sungchul, Hohng
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Analytical Assays Based on Detecting Conformational Changes of Single Molecules
ChemPhysChem, 2006AbstractOne common strategy for the detection of biomolecules is labeling either the target itself or an antibody that binds to it. Herein, a different approach, based on detecting the conformational change of a probe molecule induced by binding of the target is discussed.
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Review of single-molecule immunoassays: Non-chip and on-chip Assays
Analytica Chimica ActaEnhancing the sensitivity of immunoassays is an important requirement in the field of immunology, especially in light of rapid developments in genetic testing, making the detection of low-abundance protein biomarkers crucial. Therefore, innovations in highly sensitive immunoassays are imperative.
Yan, Su, Lei, Zhou
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Covalent Monofunctionalization of Peptide-Coated Quantum Dots for Single-Molecule Assays
Nano Letters, 2010Fluorescent probes for biological imaging of single molecules (SM) have many stringent design requirements. In the case of quantum dot (QD) probes, it remains a challenge to control their functional properties with high precision. Here, we describe the simple preparation of QDs with reduced size and monovalency.
Samuel, Clarke +5 more
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Bio-assay based on single molecule fluorescence detection in microfluidic channels
Analytical and Bioanalytical Chemistry, 2006A rapid bioassay is described based on the detection of colocalized fluorescent DNA probes bound to DNA targets in a pressure-driven solution flowing through a planar microfluidic channel. By employing total internal reflection excitation of the fluorescent probes and illumination of almost the entire flow channel, single fluorescent molecules can be ...
Hollars, Christopher W. +6 more
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Development of an On-Chip Antibiotic Permeability Assay With Single Molecule Detection Capability
IEEE Transactions on NanoBioscience, 2018Electrophysiology is the method of choice to characterize membrane channels. In this paper, we demonstrate a patch pipette based simple miniaturization that allows performing conductance measurements on a planar lipid bilayer in a microfluidic channel.
Fatma, Dogan Guzel +5 more
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