Results 171 to 180 of about 479,986 (210)

Mechanisms of human mitochondrial leaderless mRNA translation initiation. [PDF]

Nat Commun
Shen S, Xu Y, Kober DL, Wang J.
europepmc   +1 more source

Target DNA binding-induced dimerization is required for long-A prokaryotic Argonaute-mediated immunity

Swarts D, Barendse P, Drimmelen Mv, Pacesa M, Sivamaran A, Zhang D, Nickel L, Niault T, Lindhoud S, Roosjen M, Hegge J, Park K, Correia B, Oost Jvd, Joo C, Westphal A.   +15 more
europepmc   +1 more source

Single molecule enzyme-linked immunosorbent assays: Theoretical considerations [PDF]

Journal of Immunological Methods, 2012
We have developed a highly sensitive immunoassay-called digital ELISA-that is based on the detection of single enzyme-linked immunocomplexes on beads that are sealed in arrays of femtoliter wells. Digital ELISA was designed to be highly efficient in the capturing of target proteins, labeling of these proteins, and their detection in single molecule ...
David M Rissin, David R Fournier, Tomasz Piech   +2 more
exaly   +3 more sources

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Enzyme Assays for Confocal Single Molecule Spectroscopy

Current Pharmaceutical Biotechnology, 2004
During the last years confocal techniques have become increasingly popular for probing biological enzymatic reactions. In this paper we will summarize some of these methods, mainly focusing on measurement techniques suitable for analysis of freely diffusing molecules, where either the substrates or the enzymes are fluorescently labeled.
Jahnz, M., Schwille, P.
openaire   +3 more sources

Statistical Analysis of Single-Molecule Colocalization Assays

Analytical Chemistry, 2001
In chemical assays, specific molecular recognition events result in close physical proximity of two molecular species, e.g., ligands and receptors. Microscopy techniques that are able to image individual molecules allow for achieving a positional accuracy far beyond the resolution limit Therefore, independent position determination, e.g., by dual-color
Trabesinger W, Hecht B, Wild UP, Schütz GJ, Schindler H, Schmidt T   +5 more
openaire   +3 more sources

Single-molecule assay of ubiquitylation

Science Signaling, 2015
The basis of an important enzyme-target recognition strategy is revealed at the single-molecule level.
openaire   +1 more source

A single molecule assay for ultrasensitive detection of Fn14 in human serum

Analytical Biochemistry, 2019
Fibroblast growth factor inducible-14 (Fn14) is a receptor protein that plays an important role in the progression of cancer and some other diseases. Here, an ultrasensitive assay was developed for the detection of Fn14 based on a digital sandwich enzyme-linked immunosorbent bead-based assay (dELISA).
Yichen, Chen, Qiyong, Cai, Song, Liu
openaire   +2 more sources