Results 191 to 200 of about 479,986 (210)

Towards single-molecule DNA sequencing: Assays with low nonspecific adsorption

Analytical Biochemistry, 2006
New DNA sequencing techniques are currently being developed using single-molecule fluorescence-based detection of enzymatic double-strand synthesis. Such application requires surface architectures on which single-stranded templates can be immobilized.
Alexander, Krieg, Thomas, Ruckstuhl, Stefan, Seeger   +2 more
openaire   +2 more sources

Facile single-molecule pull-down assay for analysis of endogenous proteins

Physical Biology, 2019
Abstract The single-molecule pull-down (SiMPull) assay analyzes molecular complexes in physiological conditions from cell or tissue lysates. Currently the approach requires a lengthy sample preparation process, which has largely prevented the widespread adoption of this technique in bioanalysis. Here, we present a
Benjamin Croop, Kyu Young Han
openaire   +2 more sources

Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays

2018
Recent advances in single-molecule techniques allow for dynamic observations of the interactions between various protein assemblies and RNA molecules with high spatiotemporal resolution. However, it remains challenging to obtain functional eukaryotic protein complexes and cost-effective fluorescently labeled RNAs to study their interactions at the ...
Mohamed, Fareh, Chirlmin, Joo
openaire   +2 more sources

Single-Molecule Multicolor FRET Assay for Studying Structural Dynamics of Biomolecules

2016
Over the last 2 decades, single-molecule Forster resonance energy transfer (FRET) has been widely used to address important questions in molecular biology. However, a conventional approach based on a single donor-acceptor pair is not powerful enough to study complex biological systems.
S, Lee, Y, Jang, S-J, Lee, S, Hohng
openaire   +2 more sources

Ultrasensitive Automatic Detection of Small Molecules by Membrane Imaging of Single Molecule Assays

ACS Applied Materials & Interfaces, 2022
Weiya Wang, Qiaofeng Li, Dianpeng Han
exaly  

The multipurpose application of resazurin in micro-analytical techniques: Trends from the microbial, catalysis and single molecule detection assays

TrAC - Trends in Analytical Chemistry, 2023
Demian I Njoku, Wanqing Dai, Jian Lin Chen   +2 more
exaly  

Optical tweezers in single-molecule biophysics

Nature Reviews Methods Primers, 2021
CARLOS Bustamante, Yann R Chemla, Shixin Liu   +2 more
exaly  

Direct Comparison of Lysine versus Site‐Specific Protein Surface Immobilization in Single‐Molecule Mechanical Assays**

Angewandte Chemie - International Edition, 2023
Haipei Liu, Zhaowei Liu, Michael A Nash   +2 more
exaly  

Sample Preparation for Single-Molecule Enzyme Assays

2012
D.B. Craig, E.R. Nichols
openaire   +1 more source

High-Sensitivity Single Molecule Array Assays for Pathological Isoforms in Parkinson’s Disease

Clinical Chemistry, 2022
Maia Norman, Tal Gilboa, David R Walt
exaly