BOBA FRET: bootstrap-based analysis of single-molecule FRET data. [PDF]
PLoS ONE, 2013 Time-binned single-molecule Förster resonance energy transfer (smFRET) experiments with surface-tethered nucleic acids or proteins permit to follow folding and catalysis of single molecules in real-time. Due to the intrinsically low signal-to-noise ratio
Sebastian L B König +6 more
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Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation [PDF]
Nature Communications, 2023 Biomolecular condensates formed via phase separation of proteins and nucleic acids are thought to be associated with a wide range of cellular functions and dysfunctions.
Ashish Joshi +6 more
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Single-Molecule FRET: A Tool to Characterize DNA Nanostructures [PDF]
Frontiers in Molecular Biosciences, 2022 DNA nanostructures often involve temporally evolving spatial features. Tracking these temporal behaviors in real time requires sophisticated experimental methods with sufficiently high spatial and temporal resolution.
Nibedita Pal
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The potential of fluorogenicity for single molecule FRET and DyeCycling [PDF]
QRB DiscoverySingle Molecule Förster Resonance Energy Transfer (smFRET) is a popular technique to directly observe biomolecular dynamics in real time, offering unique mechanistic insight into proteins, ribozymes, and so forth.
Srijayee Ghosh, Sonja Schmid
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Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET [PDF]
Communications Biology, 2023 Single-molecule FRET experiments with human A2A adenosine receptor in its apo and agonist-bound states in lipid nanodiscs provide insights into its conformational dynamics and activation.
Ivan Maslov +18 more
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Single-Molecule FRET Imaging of Virus Spike–Host Interactions [PDF]
Viruses, 2021 As a major surface glycoprotein of enveloped viruses, the virus spike protein is a primary target for vaccines and anti-viral treatments. Current vaccines aiming at controlling the COVID-19 pandemic are mostly directed against the SARS-CoV-2 spike ...
Maolin Lu
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Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2 [PDF]
Nature Communications, 2022 Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.
Sarah Willkomm +6 more
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Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET [PDF]
BiomoleculesRiboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg2+ and their ligands playing pivotal roles in this process.
Yanyan Xue +4 more
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Single-molecule three-color FRET with both negligible spectral overlap and long observation time. [PDF]
PLoS ONE, 2010 Full understanding of complex biological interactions frequently requires multi-color detection capability in doing single-molecule fluorescence resonance energy transfer (FRET) experiments.
Sanghwa Lee, Jinwoo Lee, Sungchul Hohng
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Closing the gap between single molecule and bulk FRET analysis of nucleosomes. [PDF]
PLoS ONE, 2013 Nucleosome structure and stability affect genetic accessibility by altering the local chromatin morphology. Recent FRET experiments on nucleosomes have given valuable insight into the structural transformations they can adopt.
Alexander Gansen +4 more
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