Results 11 to 20 of about 62,512 (285)
Single-Molecule FRET of Membrane Transport Proteins. [PDF]
Chembiochem, 2021 AbstractUncovering the structure and function of biomolecules is a fundamental goal in structural biology. Membrane‐embedded transport proteins are ubiquitous in all kingdoms of life. Despite structural flexibility, their mechanisms are typically studied by ensemble biochemical methods or by static high‐resolution structures, which complicate a ...
Bartels K +3 more
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High-Resolution Single-Molecule FRET via DNA eXchange (FRET X) [PDF]
Nano Letters, 2020 ABSTRACTSingle-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object. The limited number of available FRET pair fluorophores and complicated data analysis makes it challenging to apply single-molecule FRET for structural ...
Mike Filius +3 more
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eLife, 2022 Single-molecule fluorescence spectroscopy and molecular dynamics simulations illuminate the structure and dynamics of PSD-95, a protein involved in neural plasticity.
Ivan Maslov, Jelle Hendrix
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On the statistical foundation of a recent single molecule FRET benchmark [PDF]
Nature CommunicationsAyush Saurabh +2 more
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Signal Transduction Mechanisms Quantitatively Observed One Molecule at a Time
Frontiers in Physics, 2022 Improved single-molecule methods can largely increase our understanding of underlying molecular mechanism during cellular signal transduction. In contrast to conventional bulk methods, monitoring molecules one at a time can circumvent averaging effects ...
Pei Li +4 more
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Single-Molecule Three-Color FRET [PDF]
Biophysical Journal, 2004 Fluorescence resonance energy transfer (FRET) measured at the single-molecule level can reveal conformational changes of biomolecules and intermolecular interactions in physiologically relevant conditions. Thus far single-molecule FRET has been measured only between two fluorophores.
Hohng, Sungchul +2 more
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Single‐Molecule Four‐Color FRET [PDF]
Angewandte Chemie International Edition, 2010 We developed a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopies. Real-time determination of six inter-fluorophore FRET efficiencies allowed us to probe the correlated motion of four arms of the Holliday junction.
Jinwoo, Lee +5 more
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Biosensors, 2020 Single-molecule fluorescence energy transfer (FRET) detection has become a key technique to monitor intra- and intermolecular distance changes in biological processes.
Heyjin Son +4 more
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Single-Molecule FRET Analyses of NMDA Receptors. [PDF]
Methods Mol BiolSingle-molecule fluorescence resonance energy transfer (smFRET) enables the real-time observation of conformational changes in a single protein molecule of interest. These observations are achieved by attaching fluorophores to proteins of interest in a site-specific manner and investigating the FRET between the fluorophores. Here we describe the method
Durham RJ, Jayaraman V.
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Single-Molecule FRET-Based Dynamic DNA Sensor. [PDF]
ACS Sens, 2021 Selective and sensitive detection of nucleic acid biomarkers is of great significance in early-stage diagnosis and targeted therapy. Therefore, the development of diagnostic methods capable of detecting diseases at the molecular level in biological fluids is vital to the emerging revolution in the early diagnosis of diseases. However, the vast majority
Megalathan A, Wijesinghe KM, Dhakal S.
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