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Single-Molecule FRET Analysis of Helicase Functions
2009In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales.
Eli, Rothenberg, Taekjip, Ha
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RNA Intramolecular Dynamics by Single‐Molecule FRET
Current Protocols in Nucleic Acid Chemistry, 2008AbstractInvestigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics.
Hengesbach, Martin +5 more
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Single-molecule FRET studies of Cas9 endonuclease
2019Since its discovery, the CRISPR-Cas9 system has been in the center of attention for its promising applications in genome editing. However, in order to apply this system successfully in genetic engineering, all aspects of its molecular mechanism have to be well understood. One of the best ways to investigate the intricacies of the molecular mechanism is
Viktorija, Globyte, Chirlmin, Joo
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Single-Molecule FRET-Based Multiplexed Detection
Single-molecule multiplexed detection is a high-promise toolkit for the expanding field of biosensing and molecular diagnostics. Among many single-molecule techniques available today for biomarker sensing including fluorescence, force, electrochemical, spectroscopic, barcoding, and other techniques, fluorescence-based approaches are arguably the most ...Srishty, Sethi +2 more
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Quantitative single molecule FRET efficiencies using TIRF microscopy
Faraday Discussions, 2015Förster resonance energy transfer (FRET) microscopy at the single molecule level has the potential to yield information on intra and intermolecular distances within the 2–10 nm range of molecules or molecular complexes that undergo frequent conformation changes.
Hildebrandt, Lasse +2 more
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Single-molecule FRET studies of ion channels
Progress in Biophysics and Molecular Biology, 2017Different types of fluorescence spectroscopy approaches have over the last two decades become important techniques in studies of ion channel structure and dynamics. Many fluorescence methods have been used to examine a huge variety of ion channels. Any fluorescence study of ion channels requires the presence of fluorophores, which may be intrinsic to ...
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Single-Molecule FRET Analysis of Replicative Helicases
2018Over the recent years single-molecule fluorescence resonance energy transfer (smFRET) technique has proven to be one of the most powerful tools for revealing mechanistic insights into helicase activities. Here we describe details of single-molecule FRET assays for probing DNA unwinding activities as well as functional dynamics by replicative helicases ...
Seung-Jae, Lee +2 more
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Analyzing Single Molecule FRET Trajectories Using HMM
2017Structural dynamics of biomolecules, such as proteins, plays essential roles in many biological phenomena at molecular level. It is crucial to understand such dynamics in recent biology. The single-molecule Förster resonance energy transfer (smFRET) measurement is one of few methods that enable us to observe structural changes of biomolecules in ...
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Single-Molecule FRET with Diffusion and Conformational Dynamics
The Journal of Physical Chemistry B, 2007Under relatively mild conditions, we show how one can extract information about conformational dynamics from Förster resonance energy transfer (FRET) experiments on diffusing molecules without modeling diffusion. Starting from a rigorous theory that does treat diffusion, we first examine when the single-molecule FRET efficiency distribution can be ...
Irina V, Gopich, Attila, Szabo
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Enabling multiperspective single-molecule FRET
Biophysical Journal, 2023Roman Kiselev +5 more
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