Results 31 to 40 of about 62,512 (285)
Activation-induced deoxycytidine deaminase (AID) co-transcriptional scanning at single-molecule resolution [PDF]
, 2015 Activation-induced deoxycytidine deaminase (AID) generates antibody diversity in B cells by initiating somatic hypermutation (SHM) and class-switch recombination (CSR) during transcription of immunoglobulin variable (IgV) and switch region (IgS) DNA ...
Bertram, JG +7 more
core +1 more source
A practical guide to single-molecule FRET [PDF]
Nature Methods, 2008 Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by biophysicists. We provide a practical guide to using smFRET, focusing
Rahul, Roy, Sungchul, Hohng, Taekjip, Ha
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Impact of Small Molecules on Intermolecular G-Quadruplex Formation
Molecules, 2019 We performed single molecule studies to investigate the impact of several prominent small molecules (the oxazole telomestatin derivative L2H2-6OTD, pyridostatin, and Phen-DC3) on intermolecular G-quadruplex (i-GQ) formation between two guanine-rich DNA ...
Prabesh Gyawali +5 more
doaj +1 more source
Single-Step FRET-Based Detection of Femtomoles DNA
Sensors, 2019 Sensitive detection of nucleic acids and identification of single nucleotide polymorphism (SNP) is crucial in diagnosis of genetic diseases. Many strategies have been developed for detection and analysis of DNA, including fluorescence, electrical ...
Kumar Sapkota +4 more
doaj +1 more source
Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles
Biophysical Reports, 2023 Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations. At the single-
Quinten Coucke +6 more
doaj +1 more source
Conformational dynamics of a single protein monitored for 24 hours at video rate [PDF]
, 2018 We use plasmon rulers to follow the conformational dynamics of a single protein for up to 24 h at a video rate. The plasmon ruler consists of two gold nanospheres connected by a single protein linker.
Ahijado-Guzmán, Rubén +10 more
core +3 more sources
Studying DNA Looping by Single-Molecule FRET [PDF]
Journal of Visualized Experiments, 2014 Bending of double-stranded DNA (dsDNA) is associated with many important biological processes such as DNA-protein recognition and DNA packaging into nucleosomes. Thermodynamics of dsDNA bending has been studied by a method called cyclization which relies on DNA ligase to covalently join short sticky ends of a dsDNA.
Tung T, Le, Harold D, Kim
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Protein folding studied by single-molecule FRET [PDF]
Current Opinion in Structural Biology, 2008 A complete understanding of a protein-folding mechanism requires description of the distribution of microscopic pathways that connect the folded and unfolded states. This distribution can, in principle, be described by computer simulations and theoretical models of protein folding, but is hidden in conventional experiments on large ensembles of ...
Schuler, B, Eaton, W A
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Homologous Recombination under the Single-Molecule Fluorescence Microscope [PDF]
, 2019 Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Dhakal, Soma, Gibbs, Dalton R.
core +2 more sources
Computational refinement of spectroscopic FRET measurements
Data in Brief, 2017 This article supplies raw data related to a research article entitled “Joint refinement of FRET measurements using spectroscopic and computational tools” (Kyrychenko et al., 2017) [1], in which we demonstrate the use of molecular dynamics simulations to ...
Alexander Kyrychenko +3 more
doaj +1 more source