Results 51 to 60 of about 62,512 (285)
Resolving dynamics and function of transient states in single enzyme molecules
Nature Communications, 2020 T4 Lysozyme (T4L) is a model protein whose structure is extensively studied. Here the authors combine single-molecule and ensemble FRET measurements, FRET-positioning and screening and EPR spectroscopy to study the structural dynamics of T4L and describe
Hugo Sanabria +11 more
doaj +1 more source
eLife, 2021 Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample ...
Eitan Lerner +45 more
doaj +1 more source
, 2012 Thermophilic enzymes can operate at higher temperatures but show reduced activities at room temperature. They are in general more stable during preparation and, accordingly, are considered to be more rigid in structure.
Boersch, Michael +7 more
core +1 more source
Resonance Energy Transfer [PDF]
, 2015 Resonance energy transfer, also known as Förster- or fluorescence- resonance energy transfer, or electronic energy transfer, is a photonic process whose relevance in many major areas of science is reflected both by a wide prevalence of the effect and ...
Andrews, David +3 more
core +1 more source
, 2011 In this work we monitor the catalytic mechanism of P-glycoprotein (Pgp) using single-molecule fluorescence resonance energy transfer (FRET). Pgp, a member of the ATP binding cassette family of transport proteins, is found in the plasma membrane of animal
Boersch, Michael +4 more
core +1 more source
Forster energy transfer signatures in optically driven quantum dot molecules [PDF]
, 2009 The Forster resonant energy transfer mechanism (FRET) is investigated in optically driven and electrically gated tunnel coupled quantum dot molecules. Two novel FRET induced optical signatures are found in the dressed excitonic spectrum.
B. D. Bartolo +7 more
core +1 more source
RyR1-targeted drug discovery pipeline integrating FRET-based high-throughput screening and human myofiber dynamic Ca2+ assays. [PDF]
, 2020 Elevated cytoplasmic [Ca2+] is characteristic in severe skeletal and cardiac myopathies, diabetes, and neurodegeneration, and partly results from increased Ca2+ leak from sarcoplasmic reticulum stores via dysregulated ryanodine receptor (RyR) channels ...
Bers, Donald M +6 more
core +2 more sources
Future Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy [PDF]
Biophysical Journal, 2016 Förster (or fluorescence) resonance energy transfer (FRET) is a nonradiative energy transfer process between two fluorophores located in close proximity to each other. To date, a variety of biosensors based on the principle of FRET have been developed to monitor the activity of kinases, proteases, GTPases or lipid concentration in living cells.
Eishu, Hirata, Etsuko, Kiyokawa
openaire +2 more sources
Loading Dynamics of a sliding DNA clamp [PDF]
Sliding DNA clamps are loaded at a ss/dsDNA junction by a clamp loader that depends on ATP binding for clamp opening. Sequential ATP hydrolysis results in closure of the clamp so that it completely encircles and diffuses on dsDNA.
Cho, WK +4 more
core +1 more source
36 degree step size of proton-driven c-ring rotation in FoF1-ATP synthase [PDF]
, 2009 Synthesis of the biological "energy currency molecule" adenosine triphosphate ATP is accomplished by FoF1-ATP synthase. In the plasma membrane of Escherichia coli, proton-driven rotation of a ring of 10 c subunits in the Fo motor powers catalysis in the ...
Boersch, Michael +6 more
core +2 more sources