A predicted model-aided one-step classification–multireconstruction algorithm for X-ray free-electron laser single-particle imaging [PDF]
IUCrJUltrafast, high-intensity X-ray free-electron lasers can perform diffraction imaging of single protein molecules. Various algorithms have been developed to determine the orientation of each single-particle diffraction pattern and reconstruct the 3D ...
Zhichao Jiao, Zhi Geng, Wei Ding
doaj +2 more sources
Recovery of undamaged electron-density maps in the presence of damage-induced partial coherence in single-particle imaging [PDF]
IUCrJ, 2020 Resolving the electronic structure of single biological molecules in their native state was among the primary motivations behind X-ray free-electron lasers. The ultra-short pulses they produce can outrun the atomic motion induced by radiation damage, but
Alexander Kozlov +5 more
doaj +2 more sources
Megahertz single-particle imaging at the European XFEL [PDF]
Communications Physics, 2019 The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previously possible. The European XFEL is expected
Egor Sobolev +62 more
semanticscholar +13 more sources
A predicted model-aided reconstruction algorithm for X-ray free-electron laser single-particle imaging [PDF]
IUCrJUltra-intense, ultra-fast X-ray free-electron lasers (XFELs) enable the imaging of single protein molecules under ambient temperature and pressure.
Zhichao Jiao +5 more
doaj +2 more sources
Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging. [PDF]
J Synchrotron Radiat, 2019 The development of novel single-particle imaging injection instrumentation at the European XFEL beamline SPB/SFX is presented.
Uetrecht C +6 more
europepmc +2 more sources
Theoretical study of electronic damage in single-particle imaging experiments at x-ray free-electron lasers for pulse durations from 0.1 to 10 fs. [PDF]
Physical review. E, Statistical, nonlinear, and soft matter physics, 2015 X-ray free-electron lasers (XFELs) may allow us to employ the single-particle imaging (SPI) method to determine the structure of macromolecules that do not form stable crystals.
O. Gorobtsov +3 more
semanticscholar +3 more sources
Towards single particle imaging of human chromosomes at SACLA [PDF]
, 2015 Single particle imaging (SPI) is one of the front-page opportunities which were used to motivate the construction of the first x-ray free electron lasers (XFELs).
I. Robinson +15 more
semanticscholar +3 more sources
Single Particle Imaging with the Volta Phase Plate [PDF]
Microscopy and Microanalysis, 2019 The Volta phase plate (VPP) is a remarkably simple device comprising a thin (~10 nm) amorphous carbon film positioned at the back focal plane of the objective lens (Fig. 1) [1].
R. Danev, M. Khoshouei, W. Baumeister
semanticscholar +2 more sources
Abstract P-1: Analysis of Tick-borne Encephalitis Virus Single-particle Imaging on X-ray Free-electron Laser [PDF]
International Journal of Biomedicine, 2021 Background: Tick-borne encephalitis virus (TBEV) is a dangerous human pathogen which envelope structure is already known from cryoEM study. TBEV mature viral particle size (~50 nm in diameter) makes it suitable for single-particle imaging (SPI) on X-ray ...
Grigoriy A. Armeev +6 more
doaj +1 more source
Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process. [PDF]
Cell Host Microbe, 2018 Baktash Y +3 more
europepmc +2 more sources