Results 141 to 150 of about 478,505 (298)

Indirect DNA Readout by an H-NS Related Protein: Structure of the DNA Complex of the C-Terminal Domain of Ler

, 2011
Ler, a member of the H-NS protein family, is the master regulator of the LEE pathogenicity island in virulent Escherichia coli strains. Here, we determined the structure of a complex between the DNA-binding domain of Ler (CT-Ler) and a 15-mer DNA duplex.
Cordeiro Tiago N., Bernado, P., Jesús García, Juárez Antonio, Juarez, Antonio, Bernado, Pau, Pons, Miquel, Schmidt, Holger, Antonio Juárez, Pons Miquel, Miquel Pons, Tiago N. Cordeiro, Madrid Cristina, Holger Schmidt, Cristina Madrid, Pons, M., Cordeiro, Tiago N., Bernadó Peretó, Pau, Griesinger, Christian, Tiago N Cordeiro, García, Jesús, Madrid, C., Pons Vallès, Miquel, Garcia, J., Juárez Giménez, Antonio, Juarez, A., Bernadó Pau, Pau Bernadó, Christian Griesinger, García Jesús, Cordeiro, T., Griesinger, C. ; https://orcid.org/, Madrid, Cristina, Schmidt, H., Madrid Xufré, Cristina, Schmidt Holger, Griesinger Christian   +36 more
core   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Yersinia enterocolitica provides the link between thyroid-stimulating antibodies and their germline counterparts in Graves' disease.

, 2013
Graves' disease results from thyroid-stimulating Abs (TSAbs) activating the thyrotropin receptor (TSHR). How TSAbs arise from early precursor B cells has not been established.
Atkinson, Steve, Stenkova, Anna, Dunn-Walters, Deborah, Hargreaves, Chantal E, Joshua, George W P, Buckle, Ashley M, Hampe, Christiane S, Joshua, George WP, Banga, J Paul, Wren, Brendan W, Grasso, Marco   +10 more
core   +1 more source

Nanodesigner: resolving the complex-CDR interdependency with iterative refinement

Journal of Cheminformatics
Camelid heavy-chain only antibodies consist of two heavy chains and single variable domains (VHHs), which retain antigen-binding functionality even when isolated.
Melissa Maria Rios Zertuche, Şenay Kafkas, Dominik Renn, Magnus Rueping, Robert Hoehndorf   +4 more
doaj   +1 more source

The development of single-domain VHH nanobodies that target the Candida albicans cell surface

Microbiology Spectrum
Candida albicans causes life-threatening invasive infections that are hard to diagnose and treat, with drug resistance leading to treatment failure. The goal of this study was to develop VHH (single variable domain on a heavy chain) nanobodies to detect ...
Giuseppe Buda De Cesare, Frank M. Sauer, Anna Kolecka, Aimilia A. Stavrou, Theo C. Verrips, Teun Boekhout, Edward Dolk, Carol A. Munro   +7 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Heavy Chain Only Antibodies: A New Paradigm in Personalized HER2+ Breast Cancer Therapy

BioImpacts, 2013
Unlike conventional antibodies, heavy chain only antibodies derived from camel contain a single variable domain (VHH) and two constant domains (CH2 and CH3).
Seyed Moein Moghimi, Fatemeh Rahbarizadeh, Davoud Ahmadvand, Ladan Parhamifar   +3 more
doaj   +1 more source