Results 71 to 80 of about 7,341,382 (291)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Elective single embryo transfer policy at 48/72 h: Which results after fresh transfers and frozen/thawed transfers?

, 2015
International audienceObjectives. - To evaluate our elective single embryo transfer policy performed at 48/72 h and define predictive factors of pregnancy after frozen/thawed embryo transfer. Methods.
Bottin, P., Courbiere, B., Gnisci, A., Paulmyer-Lacroix, O., Achard, Vincent, Grillo, J.-M., Paci, M., Jouve, E.   +7 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

The effect of transferring a low-quality embryo along with a high-quality embryo on the pregnancy outcome

Middle East Fertility Society Journal
Background Previous evidence suggests that low-quality embryos may send negative signals to the endometrium and affect the receptivity of the endometrium.
Xianju Huang, Xinle Lu, Xue Jiang, Ludan Chao, Xiao Wang   +4 more
doaj   +1 more source

Global variations in the uptake of single embryo transfer

, 2010
Single embryo transfer (SET) is the most effective way of reducing multiple pregnancy rates associated with assisted reproductive technology (ART). Despite published evidence suggesting that the judicious use of elective SET can lead to near-elimination ...
A. Maheshwari, S. Griffiths, Maheshwari, Abha, Bhattacharya, Siladitya, S. Bhattacharya, Griffiths, Siriol   +5 more
core   +1 more source

Double trouble: Should double embryo transfer be banned? [PDF]

, 2015
What role should legislation or policy play in avoiding the complications of in-vitro fertilization? In this article, we focus on single versus double embryo transfer, and assess three arguments in favour of mandatory single embryo transfer: risks to the
Schaefer, G. Owen, Wilkinson, Dominic, Tremellen, Kelton, Savulescu, Julian, Tremellen K., Wilkinson D., Schaefer G.O., Savulescu J.   +7 more
core   +1 more source

Shooting STAR : reinterpreting the data from the ‘Single Embryo TrAnsfeR of Euploid Embryo’ randomized clinical trial [PDF]

, 2020
Preimplantation genetic testing for aneuploidy (PGT-A) still remains controversial in clinical practice. Recently, the randomized controlled trial, ‘Single Embryo TrAnsfeR of Euploid Embryo’ (STAR) by Munné and coworkers showed a similar live birth rate
Vigano P., Somigliana E., Alteri A., Pagliardini L., Corti L., Papaleo E.   +5 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Birthweight of singletons after assisted reproduction is higher after single- than after double-embryo transfer

, 2006
BACKGROUND: Single-embryo transfer (SET) has proven efficient in reducing multiple pregnancy rates after assisted reproduction technologies (ART). This study compares outcome of singletons after SET and double-embryo transfer (DET).
Sutter, Petra De, Delbaere, Ilse, Goetgeluk, Sylvie, Temmerman, Marleen, Gerris, Jan, Verstraelen, Hans, Dhont, Marc, GOETGELUK, S, DE METSENAERE, FLEUR, der Elst, Josiane Van, De Sutter, Petra, Van der Elst, Josiane   +11 more
core   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source