Results 81 to 90 of about 411,726 (313)
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Unlocking the potential of RNA interference as a therapeutic tool [PDF]
, 2009 The existence of an intrinsic biochemical pathway enabling specified regulation of gene expression was unheard of until the final years of the last decade.
Day, Philip JR +2 more
core
siRNA-mediated knockdown of selected IGCs impairs GSC viability.
, 2017 A. Real-time qPCR analysis of IGC expression levels 24 hours after transfection of scrambled negative control siRNA (siScr) and individual siRNA candidates (1, 2, 3) for each IGC.
Robert C. Rostomily (650567) +9 more
core +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
FEBS Open Bio, EarlyView.IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo +6 more
wiley +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
Early Blockade of EphA4 Pathway Reduces Trigeminal Neuropathic Pain
Journal of Pain Research, 2020 Min-Ji Kim,* Jo-Young Son,* Jin-Sook Ju, Dong-Kuk Ahn Department of Oral Physiology, School of Dentistry, Kyungpook National University, Daegu, Korea*These authors contributed equally to this workCorrespondence: Dong-Kuk AhnDepartment of Oral Physiology,
Kim MJ, Son JY, Ju JS, Ahn DK
doaj
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli +2 more
wiley +1 more source
STUDY OF MODIFIED RECOMBINANT APOLIPOPROTEIN A-I AS A CARRIER OF SMALL INTERFERRING RNA
Сибирский научный медицинский журнал, 2019 With the widespread development of the phenomenon of RNA interference, currently, researchers are puzzled to obtain effective carriers of small interfering RNA (siRNA).
A. V. Ryabchenko +4 more
doaj +1 more source