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Single-step isolation of extracellular vesicles by size-exclusion chromatography [PDF]
Journal of Extracellular Vesicles, 2014 Background: Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of ...
Anita N. Böing +5 more
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Comparative Proteomics of Seminal Exosomes Reveals Size-Exclusion Chromatography Outperforms Ultracentrifugation [PDF]
BiomedicinesBackground: Extracellular vesicles, particularly exosomes, play a crucial role in cell–cell communication and as carriers of biomarkers. However, their use in clinical settings is limited due to a lack of standardized isolation and characterization ...
Ajaya K. Moharana +6 more
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Size Exclusion Chromatography–Mass Photometry: A New Method for Adeno-Associated Virus Product Characterization [PDF]
Cells, 2023 Over the past decade, adeno-associated viruses (AAVs) have attained significant prominence in gene therapy and genome editing applications, necessitating the development of robust and precise methodologies to ensure the quality and purity of AAV products.
Di Wu +3 more
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Universal calibration for natural polysaccharides in aqueous Size-exclusion chromatography [PDF]
E3S Web of Conferences, 2023 This article shows the validity of the Universal calibration concept in aqueous Size-exclusion chromatography for arabinogalactan and carboxymethylchitosans is presented.
Boymirzaev Azamat
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A deadenylase assay by size-exclusion chromatography. [PDF]
PLoS ONE, 2012 The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation.
Guang-Jun He, Yong-Bin Yan
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Viruses, 2021 Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu.
Hussein Kaddour +2 more
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Journal of Xenobiotics, 2022 Ecotoxicity investigations of plastic nanoparticles (NPs) should pay more attention to their ability to pass barriers, accumulate, and initiate toxicity in cells.
François Gagné
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Expression, Purification and Characterization of Functional Teduglutide Using GST Fusion System in Prokaryotic Cells [PDF]
Advanced Pharmaceutical Bulletin, 2023 Purpose: Teduglutide is the first and only FDA-approved drug for long-term treatment of short bowel syndrome (SBS). The current study aimed to present an approach for production of teduglutide using recombinant DNA technology.
Ali Akbar Alizadeh +4 more
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Partial Purification of Peroxiredoxin-2 From Porcine Skeletal Muscle
Meat and Muscle Biology, 2021 Fresh meat quality is adversely affected by protein oxidation. However, a fundamental understanding of the diverse factors that influence protein oxidation in postmortem muscle remains elusive.
Edward M;. Steadham +3 more
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Liposome retention in size exclusion chromatography
BMC Biotechnology, 2005 Background Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material.
Winterhalter Mathias +5 more
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