Results 51 to 60 of about 543,226 (367)

The specifics of small interfering RNA specificity [PDF]

Proceedings of the National Academy of Sciences, 2003
The discovery of transgene silencing in plants and double-stranded RNA (dsRNA) interference in the worm Caenorhabditis elegans has led to the latest revolution in molecular biology, RNA interference (RNAi). Over 10 years ago it was noted that several transgenic plant lines each containing the same ectopic transgene not only failed to be expressed but ...
openaire   +3 more sources

RNA Interference by Production of Short Hairpin dsRNA in ES Cells, Their Differentiated Derivatives, and in Somatic Cell Lines [PDF]

, 2003
dsRNA of several hundred nucleotides in length is effective at interfering with gene expression in mouse oocytes, pre-implantation embryos, and embryonic stem (ES) cells but is not as efficient in differentiated cell lines.
Glover, D. M., Grabarek, J. B., Plusa, B., Wianny, F., Zernicka-Goetz, M.   +4 more
core   +1 more source

Gene silencing in tick cell lines using small interfering or long double-stranded RNA [PDF]

, 2012
Gene silencing by RNA interference (RNAi) is an important research tool in many areas of biology. To effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters ...
A-C Barnard, AA Latif, AE Lew-Tabor, Alain Kohl, AM Nijhof, AM Nijhof, CL Donald, DE Brackney, EF Blouin, EM Morazzani, Esther Schnettler, F Jongejan, G Attarzadeh-Yazdi, G Barry, Gerald Barry, GJ Hannon, I Sifuentes-Romero, J Fuente de la, J Fuente de la, J Fuente de la, J Fuente de la, J Fuente de la, JC Clemens, JC Scott, JE George, JH Pedra, John K. Fazakerley, K Kiiver, KM Kocan, L Bell-Sakyi, L Bell-Sakyi, L Bell-Sakyi, L Bell-Sakyi, L Song, Lesley Bell-Sakyi, MGR Varma, P Liljestrom, P Lingor, P Willadsen, Pilar Alberdi, R Fragkoudis, R Fragkoudis, RA Zambon, S Bivalkar-Mehla, S Garcia, S Garcia, S Kurscheid, S Narasimhan, Sabine Weisheit, SM Hammond, SW Ding, TJ Kurtti, TJ Kurtti, UG Munderloh, V Nene, V Shkap, Z Zivkovic, Z Zivkovic   +57 more
core   +4 more sources

Delivering Small Interfering RNA for Novel Therapeutics [PDF]

, 2008
The gene silencing capability of RNA interference (RNAi) is being used to study individual gene's biological function and role in biochemical pathways. However, the efficacy of RNAi depends upon efficient delivery of the intermediates of RNAi, small interfering RNA (siRNA) oligonucleotides.
Patrick Y. Lu, Martin C. Woodle
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Rotavirus gene silencing by small interfering RNAs [PDF]

EMBO reports, 2002
RNA interference is an evolutionarily conserved double‐stranded RNA‐triggered mechanism for suppressing gene expression. Rotaviruses, the leading cause of severe diarrhea in young children, are formed by three concentric layers of protein, from which the spike protein VP4 projects.
Susana López, Pedro Romero, Miguel A. Déctor, Carlos F. Arias   +3 more
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Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase [PDF]

, 2015
Chronic infection of the liver by hepatitis C virus (HCV) induces a range of host factors including IFN-stimulated genes such as ISG15. ISG15 functions as an antiviral factor that limits virus replication. Previous studies have suggested that ISG15 could
Bamford, Connor G.G., Boutell, Chris, Domingues, Patricia, McLauchlan, John   +3 more
core   +2 more sources

Gauging LengthRecognition of Small Interfering RNAs

Structure, 2004
The first crystal structures of small interfering RNA (siRNA)-protein complexes revealed an elegant caliper-like principle of length selection of siRNAs.
H PARK, H WU
openalex   +3 more sources

Unzipping and binding of small interfering RNA with single walled carbon nanotube: A platform for small interfering RNA delivery [PDF]

The Journal of Chemical Physics, 2012
In an effort to design efficient platform for siRNA delivery, we combine all atom classical and quantum simulations to study the binding of small interfering RNA (siRNA) by pristine single wall carbon nanotube (SWCNT). Our results show that siRNA strongly binds to SWCNT surface via unzipping its base-pairs and the propensity of unzipping increases with
Santosh, Mogurampelly, Panigrahi, Swati, Bhattacharyya, Dhananjay, Sood, AK, Maiti, Prabal K   +4 more
openaire   +4 more sources

Single-chain antibody–delivered Livin siRNA inhibits human malignant melanoma growth in vitro and in vivo

Tumor Biology, 2017
Although gene therapy has brought new insights into the treatment of malignant melanoma, targeting delivery of nucleic acid which targets critical oncogene/anti-oncogene in vivo is still a bottleneck in the therapeutic application.
Hao Wang, Yifei Yang, Wei Wang, Bing Guan, Meng Xun, Hai Zhang, Ziling Wang, Yong Zhao   +7 more
doaj   +1 more source

Characterization of the small RNA transcriptome of the diatom, Thalassiosira pseudonana. [PDF]

PLoS ONE, 2011
This study presents the first characterization of endogenous small RNAs in a diatom, Thalassiosira pseudonana. Small RNAs act as transcriptional and translational regulators, controlling specific target genes involved in various cellular functions ...
Trina M Norden-Krichmar, Andrew E Allen, Terry Gaasterland, Mark Hildebrand   +3 more
doaj   +1 more source