Results 211 to 220 of about 15,598 (248)
Journal of Chromatography A, 2023 As an excellent fusion tag for expressing heterologous proteins, yeast SUMO (small ubiquitin-related modifier) has unique advantages such as improving solubility, promoting stability, and reducing degradation, but it lacks a simple and rapid purification method.
Zongqing Huang +6 more
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The Journal of Physical Chemistry B, 2018 Despite the growing interest in the thermal softening of proteins, the mechanistic details of it are far from understood. β-Grasp proteins have globular shape with compact structure and they are mechanically resilient. The β-clamp or mechanical clamp in them formed by the interactions between the terminal β-strands is generally associated with the ...
Shrabasti Bhattacharya +1 more
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Biosensors and Bioelectronics, 2022 Rapid and sensitive assay of pathogenic bacteria is critical for minimizing the risk of infectious diseases. Inspired by the interaction between bacteriophages and host bacteria, we obtained a gene sequence of tail fiber protein (TFP) from Pseudomonas aeruginosa (P. aeruginosa) bacteriophage. Then the gene sequence was used to express a recombinant TFP,
Yu Zhang +4 more
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Virus Research, 2003 To find cellular binding counterparts for the nucleocapsid protein (N) of Tula hantavirus (TULV), two cDNA libraries were screened using yeast two-hybrid systems based on LexA and Gal4 transcription factors. Five cDNA clones encoding SUMO-1 (Small Ubiquitin-related MOdifier, also known as sentrin) were selected in the LexA system.
Pasi Kaukinen +2 more
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Small ubiquitin-related modifier-1: Wrestling with protein regulation
The International Journal of Biochemistry & Cell Biology, 2010 Small ubiquitin-related modifier-1 (SUMO-1), a member of the SUMO family, is evolutionally conserved from yeast to humans. First identified in 1997, the active 97 amino acid protein conjugates to and modifies a wide variety of target proteins. Through post-translational SUMOylation of cellular proteins, SUMO-1 is involved in a myriad of biologically ...
Jessica Barry, Richard B. Lock
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Analytical Chemistry, 2011 Posttranslational modification by the small ubiquitin-related modifier (SUMO) is a highly regulated modification, which is often restricted to very specific cellular events. A number of analytical strategies for identification of SUMOylated proteins have been previously reported in the literature. A new screening method for SUMOylated peptides based on
Quentin Dumont +2 more
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Biochemical Society Transactions, 2007 Transcriptional activity of signal-dependent transcription factors, including nuclear receptors, relies on interacting co-regulator proteins, many of which possess protein-modifying activity. SUMOs (small ubiquitin-related modifiers) and their conjugation pathway components act as co-regulator proteins for numerous transcription factors that also are ...
Jorma J. Palvimo
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The Journal of Pharmacology and Experimental Therapeutics, 2009 The present study aimed to produce and pathophysiologically evaluate the metallothionein (MT) fusion protein. A recombinant plasmid containing DNA segment coding the pET-glutathione transferase (GST)-small ubiquitin-related modifier (SUMO)-MT fusion protein was inserted into Escherichia coli for expression.
Yadong Huang +12 more
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Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature, 2018 Athi N. Naganathan
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