Results 31 to 40 of about 6,254 (216)

CD33 forms functional dimers on cell surface to modulate Alzheimer risk [PDF]

Alzheimers Dement
Abstract Background The sialic‐acid binding immunoglobulin‐like lectin 3 receptor (Siglec‐3 / CD33) is one of the highly associated AD risk genes. Previous studies revealed that the non‐coding AD‐risk alleles (rs3865444 and rs12459419) are associated with increased total levels of CD33 expression and a higher relative expression of the long CD33M ...
Zhou Y, Satoh K, Dodd R, Enomoto M, Zhang Y, Chen F, Acheson B, Ghaffari D, Griffin J, Asher W, Manning J, Dukas G, Javitch J, Rashid M, Qamar S, Sevalle J, Bohm C, Fraser P, Bradshaw E, George‐Hyslop P.   +19 more
europepmc   +2 more sources

Single Molecule FRET: A Powerful Tool to Study Intrinsically Disordered Proteins

Biomolecules, 2018
Intrinsically disordered proteins (IDPs) are often modeled using ideas from polymer physics that suggest they smoothly explore all corners of configuration space.
Sharonda J. LeBlanc, Prakash Kulkarni, Keith R. Weninger   +2 more
doaj   +1 more source

Cooperative Analysis of Structural Dynamics in RNA-Protein Complexes by Single-Molecule Förster Resonance Energy Transfer Spectroscopy

Molecules, 2020
RNA-protein complexes (RNPs) are essential components in a variety of cellular processes, and oftentimes exhibit complex structures and show mechanisms that are highly dynamic in conformation and structure.
Nathalie Meiser, Christin Fuks, Martin Hengesbach   +2 more
doaj   +1 more source

Methods for automating the analysis of live-cell single-molecule FRET data

Frontiers in Cell and Developmental Biology, 2023
Single-molecule FRET (smFRET) is a powerful imaging platform capable of revealing dynamic changes in the conformation and proximity of biological molecules.
Jozsef Meszaros, Jozsef Meszaros, Peter Geggier, Peter Geggier, Jamie J. Manning, Jamie J. Manning, Wesley B. Asher, Wesley B. Asher, Jonathan A. Javitch, Jonathan A. Javitch, Jonathan A. Javitch, Jonathan A. Javitch   +11 more
doaj   +1 more source

Correlating membrane-protein dynamics with function: Integrating bioinformatics, molecular dynamics, and single-molecule FRET. [PDF]

Protein Sci
Abstract We present a strategy that deploys structural bioinformatics, molecular simulation, and single‐molecule Förster Resonance Energy Transfer (FRET) microscopy for observing the ligand‐dependent conformational dynamics of integral membrane proteins in situ.
Higinbotham HR, Arbour CA, Imperiali B.
europepmc   +2 more sources

Distribution of graph-distances in Boltzmann ensembles of RNA secondary structures [PDF]

, 2013
Large RNA molecules often carry multiple functional domains whose spatial arrangement is an important determinant of their function. Pre-mRNA splicing, furthermore, relies on the spatial proximity of the splice junctions that can be separated by very ...
A. Kobitski, A.M. Yoffe, A.P. Baraniak, A.X. Li, C.J. McManus, C.M. Reidys, D. Dufour, D. Leipply, D. Mathews, D.H. Mathews, H.S. Han, J.S. McCaskill, K. Darty, L.T. Fang, M. Müller, P. Clote, P. Schuster, R. Das, R. Giegerich, R. Lorenz, R. Lorenz, R. Roy, R.A. Forties, T.R. Einert, U. Gerland, U. Gerland, Y. Ding   +26 more
core   +1 more source

Preparation of ribosomes for smFRET studies: A simplified approach [PDF]

Archives of Biochemistry and Biophysics, 2016
During the past decade, single-molecule studies of the ribosome have significantly advanced our understanding of protein synthesis. The broadest application of these methods has been towards the investigation of ribosome conformational dynamics using single-molecule Förster resonance energy transfer (smFRET).
Bassem, Shebl, Drew E, Menke, Min, Pennella, Raghav R, Poudyal, Donald H, Burke, Peter V, Cornish   +5 more
openaire   +2 more sources

DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning

eLife, 2020
Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for studying the structure and dynamics of biomolecules. The development of high throughput methodologies and the growth of commercial instrumentation have outpaced the ...
Johannes Thomsen, Magnus Berg Sletfjerding, Simon Bo Jensen, Stefano Stella, Bijoya Paul, Mette Galsgaard Malle, Guillermo Montoya, Troels Christian Petersen, Nikos S Hatzakis   +8 more
doaj   +1 more source

BOBA FRET: bootstrap-based analysis of single-molecule FRET data. [PDF]

PLoS ONE, 2013
Time-binned single-molecule Förster resonance energy transfer (smFRET) experiments with surface-tethered nucleic acids or proteins permit to follow folding and catalysis of single molecules in real-time. Due to the intrinsically low signal-to-noise ratio
Sebastian L B König, Mélodie Hadzic, Erica Fiorini, Richard Börner, Danny Kowerko, Wolf U Blanckenhorn, Roland K O Sigel   +6 more
doaj   +1 more source

Activation-induced deoxycytidine deaminase (AID) co-transcriptional scanning at single-molecule resolution [PDF]

, 2015
Activation-induced deoxycytidine deaminase (AID) generates antibody diversity in B cells by initiating somatic hypermutation (SHM) and class-switch recombination (CSR) during transcription of immunoglobulin variable (IgV) and switch region (IgS) DNA ...
Bertram, JG, Chaurasiya, KR, Goodman, MF, Jaszczur, M, Mak, CH, Pham, P, Rueda, DS, Senavirathne, G   +7 more
core   +1 more source