Results 41 to 50 of about 6,254 (216)

Riboswitch Structure and Dynamics by smFRET Microscopy [PDF]

, 2014
Riboswitches are structured noncoding RNA elements that control the expression of their embedding messenger RNAs by sensing the intracellular concentration of diverse metabolites. As the name suggests, riboswitches are dynamic in nature so that studying their inherent conformational dynamics and ligand-mediated folding is important for understanding ...
Krishna C, Suddala, Nils G, Walter
openaire   +2 more sources

Conformational Selection and Induced Fit: The Behavior of Two Homologous Proteases

ChemistryEurope, 2023
Protein‐ligand interactions are crucial for many cellular processes, with details of the binding mechanism being discussed as essential for biological functions.
Hannah Maus, Gerald Hinze, Stefan J. Hammerschmidt, Prof. Dr. Tanja Schirmeister, Prof. Dr. Thomas Basché   +4 more
doaj   +1 more source

The telomerase essential N-terminal domain promotes DNA synthesis by stabilizing short RNA-DNA hybrids. [PDF]

, 2015
Telomerase is an enzyme that adds repetitive DNA sequences to the ends of chromosomes and consists of two main subunits: the telomerase reverse transcriptase (TERT) protein and an associated telomerase RNA (TER). The telomerase essential N-terminal (TEN)
Akiyama, Benjamin M, Parks, Joseph W, Stone, Michael D   +2 more
core   +2 more sources

Exploring Intra- and Inter-Regional Interactions in the IDP α-Synuclein Using smFRET and MD Simulations

Biomacromolecules, 2023
Theoretical concepts from polymer physics are often used to describe intrinsically disordered proteins (IDPs). However, amino acid interactions within and between regions of the protein can lead to deviations from typical polymer scaling behavior and ...
Gobert Heesink, Mirjam J Marseille, M. Fakhree, Mark D. Driver, Kirsten A van Leijenhorst-Groener, P. Onck, C. Blum, M. Claessens   +7 more
semanticscholar   +1 more source

pyFRET: A Python Library for Single Molecule Fluorescence Data Analysis [PDF]

, 2016
Single molecule Förster resonance energy transfer (smFRET) is a powerful experimental technique for studying the properties of individual biological molecules in solution.
Jackson, SE, Klenerman, D, Murphy, RR
core   +7 more sources

Quantitative comparison between sub-millisecond time resolution single-molecule FRET measurements and 10-second molecular simulations of a biosensor protein.

PLoS Computational Biology, 2020
Molecular Dynamics (MD) simulations seek to provide atomic-level insights into conformationally dynamic biological systems at experimentally relevant time resolutions, such as those afforded by single-molecule fluorescence measurements.
Dylan Girodat, Avik K Pati, Daniel S Terry, Scott C Blanchard, Karissa Y Sanbonmatsu   +4 more
doaj   +1 more source

Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation [PDF]

, 2009
Determining the mechanism by which transfer RNAs (tRNAs) rapidly and precisely transit through the ribosomal A, P and E sites during translation remains a major goal in the study of protein synthesis.
Chris H. Wiggins, Jake M. Hofman, Jingyi Fei, Jonathan E. Bronson, Moran, Rathi L. Srinivas, Ruben L. Gonzalez   +6 more
core   +4 more sources

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe, Abbe, Ai, Airy, Alivisatos, Alivisatos, Ando, Aoki, Auksorius, Auzel, Auzel, Axelrod, Axelrod, Bacskai, Bader, Bader, Balse, Barroso, Bastiaens, Bates, Beaudouin, Beaurepaire, Becker, Benninger, Berezin, Berney, Betzig, Bianchini, Biju, Binnemans, Borst, Brakemann, Bretschneider, Bunt, Campagnola, Campagnola, Campbell, Cantor, Carrero, Chalfie, Chalfie, Chatterjee, Chen, Chen, Clapp, Clapp, Clapp, Cole, Coons, Dahan, Dedecker, Del Pozo, Deng, Denk, Diaspro, Diaspro, Dickson, Digman, Drobizhev, Drummen, Dundr, Dundr, Dunn, Dunn, Dunn, Durr, Dyba, Ehrlicher, Einstein, Elangovan, Elder, Ellenberg, Elsner, Feige, Fereidouni, Festenstein, Frommer, Förster, Förster, Gamelin, Garcia-Mira, Garcia-Parajo, Gerlich, Giese, Giordano, Goodson, Gopich, Gordon, Gregor P. C. Drummen, Grotjohann, Gu, Gunnlaugsson, Gurskaya, Gustafsson, Göppert-Mayer, Ha, Haugland, Heimstädt, Hellen C. Ishikawa-Ankerhold, Hemmila, Hiller, Hitakomate, Hofkens, Hofmann, Houtsmuller, Houtsmuller, Howell, Hu, Hu, Huang, Ishihama, Jacobson, Jares-Erijman, Jiang, Johannes, Johnson, Jonkman, Jose, Jovin, Jovin, Kaiser, Kambara, Kapustina, Karasawa, Kardash, Kasha, Kawashima, Kenworthy, Kerppola, Kerppola, Kerppola, Kerppola, Kimura, Kinoshita, Klonis, Kner, Knoblauch, Konig, Koppel, Koster, Kota, Koushik, Kremers, Kremers, Kuningas, Kuningas, Kuningas, Laforge, Lakowicz, Lakowicz, Larson, Lee, Lichtman, Liebman, Lippincott-Schwartz, Liu, Llères, Loura, Lukyanov, Lundin, Mansfield, Mathew-Fenn, Mattheyses, Matz, Medintz, Mills, Mitchell, Miyawaki, Miyawaki, Moerner, Moneron, Monetta, Mueller, Mueller, Nagai, Nehls, Ormö, Paddock, Parsons, Patterson, Patterson, Patterson, Pawley, Pawley, Persechini, Peters, Phair, Phair, Phillips, Piston, Piston, Planck, Pockwinse, Politz, Poo, Puliti, Rajaram, Rantanen, Reinhard, Reits, Reymann, Richard Ankerhold, Richards, Riven, Rizzo, Rizzo, Robinson, Roduner, Rotblat, Roy, Rust, Sakon, Sbalzarini, Schawlow, Schönle, Seabrooke, Seah, Selvin, Shaner, Shav-Tal, Shaw, Shchyolkina, Shimomura, Shimomura, Shroff, Shu, Shyu, Smith, So, Solon, Soukka, Soumpasis, Sprague, Sprague, Stasevich, Stelzer, Stepanenko, Stokes, Stryer, Stryer, Subach, Sun, Suyver, Swaminathan, Swift, Sönnichsen, Tagawa, Taylor, Thaler, Tian, Tian, Tinnefeld, Tisler, Tocanne, Tomosugi, Tripathi, Tsien, Tsien, Uphoff, Valentin, van Driel, Van Munster, Van Munster, van Munster, van Rheenen, van Royen, van Royen, van Royen, Varghese, Venetta, Vogelsang, von Wichert, Vosch, Wallrabe, Wang, Wang, Wang, Wang, Wang, Wang, Welch, White, Williams, Willig, Wouters, Wouters, Wouters, Wouters, Wright, Xia, Yang, Youvan, Zhang, Zhang, Zimmer, Zipfel   +295 more
core   +2 more sources

8-spot smFRET analysis using two 8-pixel SPAD arrays [PDF]

SPIE Proceedings, 2013
Single-molecule Förster resonance energy transfer (smFRET) techniques are now widely used to address outstanding problems in biology and biophysics. In order to study freely diffusing molecules, current approaches consist in exciting a low concentration (
INGARGIOLA, ANTONINO, PANZERI, FRANCESCO, N. Sarkhosh, GULINATTI, ANGELO, RECH, IVAN, GHIONI, MASSIMO ANTONIO, S. Weiss, X. Michalet   +7 more
openaire   +2 more sources

Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state

Nature Communications, 2021
Here, the authors use smFRET to assess the structural dynamics of metabotropic glutamate receptor mGlu2 and show that a positive allosteric modulator or the Gi protein stabilize mGlu2 in the glutamate-induced active state, leading to the full activation ...
Anne-Marinette Cao, Robert B. Quast, Fataneh Fatemi, Philippe Rondard, Jean-Philippe Pin, Emmanuel Margeat   +5 more
doaj   +1 more source