Results 111 to 120 of about 3,191 (253)
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Binding of the exocyst complex to the SNARE regulator Sec1
, 2020 The process of intracellular membrane fusion relies on several protein families, each playing multiple roles in assisting vesicle docking and SNARE assembly.
Forson, Jacqueline
core
Size of Supramolecular SNARE Complex: Membrane-Directed Self-Assembly
, 2016 Full length v-SNARE protein in lipid vesicles when exposed to t-SNARE-reconstituted lipid membrane results in the self-assembly of a t-/v-SNARE complex in a ring pattern, forming pores, and establishing continuity between the opposing bilayers.
Aleksandar Jeremic (2655604) +2 more
core +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Frontiers in Immunology, 2018 Vesicle-associated V-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins and target membrane-associated T-SNAREs (syntaxin 4 and SNAP-23) assemble into a core trans-SNARE complex that mediates membrane fusion during ...
Yoosoo Yang +13 more
doaj +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Dimeric Peptide Inhibitor Interacting with Neuronal-SNARE Complex
, 2020 The neuronal SNAREs form a protein complex that mediates Ca2⁺-triggered neurotransmitter release into the synaptic cleft. t-SNAREs (Syntaxin-1 and SNAP-25) and v-SNARE (Synaptobrevin-2) interact with each other to form a SNARE complex.
Jihye, Choi
core
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Intravenous thrombolysis (IVT) before thrombectomy for ischemic stroke may alter clot structure and procedural performance. We investigated how IVT relates to thrombectomy metrics across stroke etiologies. Methods We performed a time‐to‐event analysis of consecutive patients with anterior circulation large vessel occlusion (acLVO ...
Annahita Sedghi +8 more
wiley +1 more source
Electrostatic interaction energies between the SNARE complex and fusing membranes.
, 2013 (A) Schematic diagram and definitions of intermolecular interaction energies presented in (B) and (C). In (A) through (C), only the SNARE core complex is considered.
Ting Guo (210829) +2 more
core +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Variants in SLC6A1, encoding the GABA transporter 1 (GAT‐1), cause epilepsy, autism spectrum disorder, and developmental delay via loss of GABA uptake, impaired trafficking, and ER retention. We previously found that 4‐Phenylbutyrate (PBA), an FDA‐approved drug, restores GABA uptake and reduces seizures in SLC6A1‐related disorders ...
Melissa B. DeLeeuw +5 more
wiley +1 more source