Results 61 to 70 of about 1,382 (128)

Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways

Microbial Biotechnology, Volume 19, Issue 4, April 2026.
Engineered Saccharomyces cerevisiae reveals how cell wall remodelling and membrane trafficking coordinate extracellular vesicle biogenesis. Interferon overexpression and chs3 deletion enhance EV secretion and reshape SNARE‐, Rab‐ and ESCRT‐associated pathways, establishing yeast as a versatile platform for EV engineering and therapeutic cargo loading ...
Yueyan Li, XiaoRan Ma, Lichao Zhang, Ning Cao, Zhibo Li, Ruixin Khoo, Mei Wang, Changyan Li, Deping Hua, Xintian Zheng, Jinhai Huang, Lilin Zhang   +11 more
wiley   +1 more source

Structural Basis for Regulation of ESCRT-III Complexes by Lgd

Cell Reports, 2017
The ESCRT-III complex induces outward membrane budding and fission through homotypic polymerization of its core component Shrub/CHMP4B. Shrub activity is regulated by its direct interaction with a protein called Lgd in flies, or CC2D1A or B in humans ...
Brian J. McMillan, Christine Tibbe, Andrew A. Drabek, Tom C.M. Seegar, Stephen C. Blacklow, Thomas Klein   +5 more
doaj   +1 more source

Host ESCRT proteins are required for bromovirus RNA replication compartment assembly and function.

PLoS Pathogens, 2015
Positive-strand RNA viruses genome replication invariably is associated with vesicles or other rearranged cellular membranes. Brome mosaic virus (BMV) RNA replication occurs on perinuclear endoplasmic reticulum (ER) membranes in ~70 nm vesicular ...
Arturo Diaz, Jiantao Zhang, Abigail Ollwerther, Xiaofeng Wang, Paul Ahlquist   +4 more
doaj   +1 more source

Analysis of Genetic Variation of Rice Straw Characteristics and Its Influence on Biomass

Plant Direct, Volume 10, Issue 1, January 2026.
ABSTRACT Rice straw is a key source of lignocellulosic biomass. GWAS can be used to identify genetic loci controlling stem morphological traits that influence biomass. This study aimed to investigate the genotypic diversity of rice straw internodes through GWAS, using 34,232 single‐nucleotide polymorphic sites with a minor allelic frequency (MAF ...
Mahta Mohamadiaza, Naser Farrokhi, Asadollah Ahmadikhah, Pär K. Ingvarsson, Mehdi Jahanfar   +4 more
wiley   +1 more source

Cellular Coordination of Transcription Factors and Regulatory Genes for Cryptococcal Virulence

Cellular Microbiology, Volume 2026, Issue 1, 2026.
Cryptococcus neoformans is an opportunistic pathogen belonging to the family Cryptococcaceae, capable of causing systemic and invasive fungal infections in humans and animals. The infection caused by Cryptococcus is lethal primarily in immunocompromised individuals due to the overwhelming expression of virulence factors that drive cryptococcosis in ...
Olufemi S. Folorunso, Olihile M. Sebolai, Sanket Kaushik   +2 more
wiley   +1 more source

From Mitochondria to Immunity: The Emerging Roles of Mitochondria‐Derived Vesicles and Small Extracellular Vesicles in Cellular Communication and Disease

Journal of Extracellular Vesicles, Volume 14, Issue 11, November 2025.
ABSTRACT According to the endosymbiotic theory of mitochondrial origin, an α‐proteobacterium entered a prokaryotic cell and, through symbiosis, evolved into the mitochondria—the powerhouse of the cell. Like other bacteria, the α‐proteobacteria generate their own extracellular vesicles (EVs), a trait that was passed onto the mitochondria, enabling them ...
Rostyslav Horbay, Vasyl Syrvatka, Artem Bedzay, Mikaela van der Merwe, Dylan Burger, Shawn T. Beug   +5 more
wiley   +1 more source

Molecular Mechanisms of Extracellular Vesicle Biogenesis and Their Impact on the Design of Custom EVs

Advanced Healthcare Materials, Volume 14, Issue 23, September 8, 2025.
Extracellular vesicles (EVs) are pivotal mediators of intercellular communication and disease, yet the fundamental mechanisms controlling their biogenesis and cargo selection remain unclear. This limitation hinders their diagnostic utility and therapeutic development.
Luís Carvalho Ferraz, Paulo Pereira, João Vasco Ferreira   +2 more
wiley   +1 more source

The quest for the best target genes for RNAi‐mediated pest control

Insect Molecular Biology, Volume 34, Issue 4, Page 505-517, August 2025.
A major challenge for RNAi mediated pest control is the identification of the most effective RNAi target genes from the pool of ~37% of all genes that are essential. Effective RNAi target genes are predominantly highly conserved genes serving basic cellular functions such as protein degradation.
Doga Cedden, Gregor Bucher
wiley   +1 more source

Structural Analysis of the ESCRT-III Regulator Lethal(2) Giant Discs/Coiled-Coil and C2 Domain-Containing Protein 1 (Lgd/CC2D1)

Cells
Members of the LGD/CC2D1 protein family contain repeats of the family-defining DM14 domains. Via this domain, they interact with members of the CHMP family, which are essential for the ESCRT machinery-mediated formation of intraluminal vesicles during ...
Thomas Breuer, Christine Tibbe, Tobias Troost, Thomas Klein   +3 more
doaj   +1 more source

Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA

Insect Molecular Biology, Volume 34, Issue 4, Page 581-592, August 2025.
Colorado potato beetle's RNA interference (RNAi) response was enhanced when insecticidal dsactin was consumed at high temperatures. The enhanced RNAi outcomes at high temperatures were not a function of dose or the expression of core RNAi genes. The potato cultivar consumed by Colorado potato beetle larvae did not affect RNAi outcomes, but larvae did ...
Mike Darrington, Jason Solocinski, Sophia K. Zhou, Melise C. Lecheta, Subba Reddy Palli, Yolanda H. Chen, Nicholas M. Teets   +6 more
wiley   +1 more source