Results 101 to 110 of about 15,972 (195)

Structural Variations Contribute to Subspeciation and Yield Heterosis in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Yield heterosis has been extensively exploited in hybrid breeding, with intersubspecific hybrids often exhibiting the most pronounced effects. However, developing elite hybrids remains a laborious and time‐consuming process. The genetic basis of heterosis has been debated for over a century, hindered largely by the lack of high‐quality genomes.
Zhiwu Dan, Yunping Chen, Wenchao Huang
wiley   +1 more source

Optimized Method for High-Quality Isolation of Single-Nuclei From Mosquito Fat Body for RNA Sequencing

Bio-Protocol
Single-cell and single-nucleus RNA sequencing are revolutionizing our understanding of cellular biology. The identification of molecular markers, single-cell transcriptomic profiling, and differential gene expression at the cellular level has revealed ...
Stephanie de Carvalho, Colton McNinch, Carolina Barillas-Mury   +2 more
doaj   +1 more source

Single‐Cell CRISPR: An Efficient Strategy for Decoding Plant Cis‐Regulatory Complexity

Plant Biotechnology Journal, EarlyView.
ABSTRACT The generation of complex traits involves the coordinated interplay of multiple gene networks. Elucidating the function of transcriptional cis‐regulatory elements (CREs) in regulating gene expression is crucial for understanding complex regulatory pathways and improving our ability to modify macro‐phenotypes.
Yuanhang Zhang, Xuanxuan Luo, Hongbin Li, Shuangxia Jin, Xianlong Zhang, Maojun Wang   +5 more
wiley   +1 more source

Spatiotemporal Metabolome and Single‐Nucleus Transcriptome Integration Illuminates an Auxin Gradient Orchestrated by NtTAC1 Underlying Leaf Angle Regulation in Tobacco

Plant Biotechnology Journal, EarlyView.
Proposed model of TAC1‐mediated leaf angle regulation in tobacco. ABSTRACT Plant architecture is key to crop yield, with leaf angle being critical for high‐density cultivation. Although TAC1 represents a promising regulator of leaf angle for breeding, its molecular mechanism remains poorly understood, particularly at single‐nucleus resolution. Here, we
Lin Wang, Junping Gao, Chen Wang, Guoyun Xu, Zhen Ma, Shuaibin Wang, Zhaopeng Luo, Mingzhu Wu, Jianfeng Zhang, Jun Yang, Peijian Cao, Xiaodong Xie   +11 more
wiley   +1 more source

Mitochondrial control of ciliary gene expression and structure in striatal neurons

The Journal of Physiology, EarlyView.
Abstract figure legend Neurons drive animal behaviour by receiving and transmitting information and require energy, primarily supplied by mitochondria, to function. Additionally, neurons need to sense environmental changes to adapt, a function that is locally played by the primary cilia.
Dogukan H. Ulgen, Alessandro Chioino, Olivia Zanoletti, Albert Quintana, Elisenda Sanz, Carmen Sandi   +5 more
wiley   +1 more source

Identification of epileptic hippocampal sclerosis related genes through bulk and single-nucleus RNA sequencing datasets

Cellular and Molecular Life Sciences
Background Hippocampal sclerosis is one of the most common pathological alterations in drug-refractory epilepsy. However, the exact causes and molecular mechanisms of its formation remain unknown. Methods We constructed a hippocampal sclerosis score (HSS)
Jincheng Wang, Zhongyu Zhou, Die Wu, Yiqiao Zeng, Hanyu Huang, Qigang Zhou, Fan Meng   +6 more
doaj   +1 more source

Experimental models of cerebral small vessel disease: Physiological constraints, translational challenges and future directions

The Journal of Physiology, EarlyView.
Abstract figure legend Overview of cerebral small vessel disease (cSVD) pathophysiology and current modelling challenges. Left: clinical burden and key unresolved research questions. Right: neurovascular unit (NVU) architecture highlighting sites of cSVD pathology across arterioles, capillaries and venules, including interactions between endothelial ...
Sophie Beaumont, Tanya Singh, Luana Campos Soares, Mootaz M. Salman   +3 more
wiley   +1 more source

Comparative analysis of microRNAs in bovine colostrum and neonatal calf blood

Journal of the Science of Food and Agriculture, Volume 106, Issue 10, Page 5799-5809, 15 August 2026.
Abstract BACKGROUND MicroRNAs (miRNAs) are short non‐coding RNAs that participate in a wide range of biological processes. MiRNAs are present in high concentrations in colostrum, and these may be potentially transferred to newborn calves being fed colostrum.
Do T Hue, Kiro Petrovski, Yan Ren, John L Williams, Tong Chen, Wai Y Low, Thien D Van, Cynthia D K Bottema   +7 more
wiley   +1 more source

Tumor‐educated platelets in cancer diagnostics and prognostics: A critical appraisal and roadmap for clinical translation

International Journal of Cancer, Volume 159, Issue 2, Page 302-319, 15 July 2026.
Abstract Tumor‐educated platelets (TEPs) are emerging as a compelling frontier in liquid biopsy, functioning as dynamic, systemic sensors that sequester and process tumor‐derived biomolecules. This interaction imprints an integrated molecular signature of malignancy—spanning the transcriptome, proteome, lipidome, and crucially, the captured genome ...
Whi‐An Kwon, Min‐Kyung Lee, Eunyong Ahn, Heeyeon Kim, Yong Sang Song, Taejin Ahn   +5 more
wiley   +1 more source

An improved nuclei isolation protocol from leaf tissue for single-cell transcriptomics.

PLoS ONE
The study of plant biology has traditionally focused on investigations conducted at the tissue, organ, or whole plant level. However, single-cell transcriptomics has recently emerged as an important tool for plant biology, enabling researchers to uncover
Gabriela Madrid, Gabriel Angelo Saraiva Raimundo, Fabian Andres Reyes, Edgard Augusto de Toledo Picoli, Marcio F R Resende, Kelly M Balmant   +5 more
doaj   +1 more source