TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation [PDF]
Nature Communications, 2021 The correct assembly and recycling of the multicomponent spliceosome remains largely elusive. Here, the authors show that a previously uncharacterized protein TSSC4 associates with de novo formed spliceosomal U5 snRNP as well as with a post-splicing U5 ...
Klára Klimešová +6 more
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The 7SK/P-TEFb snRNP controls ultraviolet radiation-induced transcriptional reprogramming
Cell Reports, 2021 Summary: Conversion of promoter-proximally paused RNA polymerase II (RNAPII) into elongating polymerase by the positive transcription elongation factor b (P-TEFb) is a central regulatory step of mRNA synthesis. The activity of P-TEFb is controlled mainly
Cécilia Studniarek +5 more
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The mechanism of release of P-TEFb and HEXIM1 from the 7SK snRNP by viral and cellular activators includes a conformational change in 7SK. [PDF]
PLoS ONE, 2010 The positive transcription elongation factor, P-TEFb, is required for the production of mRNAs, however the majority of the factor is present in the 7SK snRNP where it is inactivated by HEXIM1. Expression of HIV-1 Tat leads to release of P-TEFb and HEXIM1
Brian J Krueger +3 more
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The Role of the U5 snRNP in Genetic Disorders and Cancer
Frontiers in Genetics, 2021 Pre-mRNA splicing is performed by the spliceosome, a dynamic macromolecular complex consisting of five small uridine-rich ribonucleoprotein complexes (the U1, U2, U4, U5, and U6 snRNPs) and numerous auxiliary splicing factors.
Katherine A. Wood +5 more
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An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses. [PDF]
PLoS Pathogens, 2015 Adeno-associated viruses (AAV) have evolved to exploit the dynamic reorganization of host cell machinery during co-infection by adenoviruses and other helper viruses.
Claire A Schreiber +9 more
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An alternative D. melanogaster 7SK snRNP [PDF]
BMC Molecular and Cell Biology, 2021 Abstract Background The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa.
Duy Nguyen +6 more
openaire +3 more sources
A novel intra-U1 snRNP cross-regulation mechanism: alternative splicing switch links U1C and U1-70K expression. [PDF]
PLoS Genetics, 2013 The U1 small nuclear ribonucleoprotein (snRNP)-specific U1C protein participates in 5' splice site recognition and regulation of pre-mRNA splicing. Based on an RNA-Seq analysis in HeLa cells after U1C knockdown, we found a conserved, intra-U1 snRNP cross-
Tanja Dorothe Rösel-Hillgärtner +5 more
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Conditional U1 gene silencing in Toxoplasma gondii [PDF]
, 2015 The functional characterisation of essential genes in apicomplexan parasites, such as Toxoplasma gondii or Plasmodium falciparum, relies on conditional mutagenesis systems. Here we present a novel strategy based on U1 snRNP-mediated gene silencing.
Blackman, Michael J. +10 more
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Plant snRNP Biogenesis: A Perspective from the Nucleolus and Cajal Bodies
Frontiers in Plant Science, 2018 Small nuclear ribonucleoproteins (snRNPs) are protein–RNA complexes composed of specific snRNP-associated proteins along with small nuclear RNAs (snRNAs), which are non-coding RNA molecules abundant in the nucleus.
Misato Ohtani, Misato Ohtani
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Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries [PDF]
, 1993 The nuclear topography of splicing snRNPs, mRNA transcripts and chromosome domains in various mammalian cell types are described. The visualization of splicing snRNPs, defined by the Sm antigen, and coiled bodies, revealed distinctly different ...
A Monneron +64 more
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