Results 61 to 70 of about 13,335 (199)

Acetylation by the transcriptional coactivator Gcn5 plays a novel role in co-transcriptional spliceosome assembly. [PDF]

PLoS Genetics, 2009
In the last several years, a number of studies have shown that spliceosome assembly and splicing catalysis can occur co-transcriptionally. However, it has been unclear which specific transcription factors play key roles in coupling splicing to ...
Felizza Q Gunderson, Tracy L Johnson
doaj   +1 more source

A unified mechanism for intron and exon definition and back-splicing. [PDF]

, 2019
The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E complex assembled on introns, providing a view of the ...
Cui, Yanxiang, Das, Rhiju, Espinosa, Sara, Hansen, Kirk C, Hill, Ryan C, Issaian, Aaron, Kappel, Kalli, Li, Xueni, Liu, Shiheng, Shi, Shasha, Zhang, Lingdi, Zhao, Rui, Zhou, Z Hong   +12 more
core  

Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. [PDF]

, 1994
To study the functions of heterogeneous nuclear ribonucleoproteins (hnRNPs), we have characterized nuclear polyadenylated RNA-binding (Nab) proteins from Saccharomyces cerevisiae.
Datar, KV, Paddy, MR, Swanson, MS, Swedlow, JR, Wilson, SM   +4 more
core   +1 more source

Human Cyclophilins—An Emerging Class of Drug Targets

Medicinal Research Reviews, Volume 46, Issue 2, Page 475-512, March 2026.
ABSTRACT Cyclophilins are a family of enzymes with peptidyl‐prolyl isomerase activity found in all cells of all organisms. To date, 17 cyclophilin isoforms have been identified in the human body, participating in diverse biological processes. Consequently, cyclophilins have emerged as promising targets for drug development to address a wide array of ...
Katarina Jurkova, Hana Navratilova, Kamil Musilek, Ondrej Benek   +3 more
wiley   +1 more source

JMJD6 cleaves MePCE to release positive transcription elongation factor b (P-TEFb) in higher eukaryotes

eLife, 2020
More than 30% of genes in higher eukaryotes are regulated by promoter-proximal pausing of RNA polymerase II (Pol II). Phosphorylation of Pol II CTD by positive transcription elongation factor b (P-TEFb) is a necessary precursor event that enables ...
Schuyler Lee, Haolin Liu, Ryan Hill, Chunjing Chen, Xia Hong, Fran Crawford, Molly Kingsley, Qianqian Zhang, Xinjian Liu, Zhongzhou Chen, Andreas Lengeling, Kathrin Maria Bernt, Philippa Marrack, John Kappler, Qiang Zhou, Chuan-Yuan Li, Yuhua Xue, Kirk Hansen, Gongyi Zhang   +18 more
doaj   +1 more source

ALS-linked FUS mutants affect the localization of U7 snRNP and replication-dependent histone gene expression in human cells

Scientific Reports, 2021
Genes encoding replication-dependent histones lack introns, and the mRNAs produced are a unique class of RNA polymerase II transcripts in eukaryotic cells that do not end in a polyadenylated tail.
Ankur Gadgil, Agnieszka Walczak, Agata Stępień, Jonas Mechtersheimer, Agnes Lumi Nishimura, Christopher E. Shaw, Marc-David Ruepp, Katarzyna Dorota Raczyńska   +7 more
doaj   +1 more source

In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles [PDF]

, 2002
The NHPX protein is a nucleolar factor that binds directly to a conserved RNA target sequence found in nucleolar box C/D snoRNAs and in U4 snRNA. Using enhanced yellow fluorescent protein (EYFP)– and enhanced cyan fluorescent protein–NHPX fusions, we ...
Lamond, Angus I., Leung, Anthony K. L.
core   +5 more sources

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, Volume 16, Issue 2, Page 299-313, February 2026.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

Anti-U5 Small Nuclear Ribonucleoprotein (snRNP) Antibodies: A Rare Anti-U snRNP Specificity

Clinical Immunology and Immunopathology, 1996
We surveyed for autoantibodies to the U small nuclear ribonucleoproteins (snRNPs) in sera from 1171 patients with various connective tissue diseases by immunoprecipitation assay. We found serum, termed LaJ, which principally recognized the U5 snRNP from one patient with systemic sclerosis-polymyositis overlap syndrome. Anti-LaJ serum immunoprecipitated
Y, Okano, I N, Targoff, C V, Oddis, T, Fujii, M, Kuwana, K, Tsuzaka, M, Hirakata, T, Mimori, J, Craft, T A, Medsger   +9 more
openaire   +2 more sources

Reconstitution of a functional 7SK snRNP [PDF]

Nucleic Acids Research, 2017
The 7SK small nuclear ribonucleoprotein (snRNP) plays a central role in RNA polymerase II elongation control by regulating the availability of active P-TEFb. We optimized conditions for analyzing 7SK RNA by SHAPE and demonstrated a hysteretic effect of magnesium on 7SK folding dynamics including a 7SK GAUC motif switch.
Brogie, John E., Price, David H.
openaire   +2 more sources