Results 51 to 60 of about 11,718,333 (235)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Forum: Qualitative Social Research, 2008 RATNER's "Epistemological, Social, and Political Conundrums in Social Constructionism" provides ample illustration of how a grossly negligent, read both limited and limiting, exegesis of social constructionism has the demonstrable effect of installing ...
Pascal Dey
doaj
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Epistemological, Social, and Political Conundrums in Social Constructionism
Forum: Qualitative Social Research, 2006 This article critiques the central premise of social constructionism, namely that groups of people freely construct beliefs about things and that beliefs are "local truths" which must be honored by outsiders and cannot be evaluated by external criteria ...
Carl Ratner
doaj
Discourse studies: Between social constructionism and linguistics. A critical overview
Topics in Linguistics, 2019 This paper gives a critical overview of the analytical approaches dominating the field of discourse studies in the last three decades, from the perspective of their philosophical and formative bases: social constructionism and linguistics.
Cap Piotr
doaj +1 more source
What is Constructionism in Psychiatry? From Social Causes to Psychiatric Classification
Frontiers in Psychiatry, 2016 It is common to note that social environment and cultural formation shape mental disorders. The details of this claim are, however, not well understood. The paper takes a look at the claim that culture has an impact on psychiatry from the perspective of ...
Raphael evan Riel
doaj +1 more source
Solution focused approach. An attempt to adapt the method to the process of social rehabilitation
Polish Journal of Social Rehabilitation, 2020 In the search for an answer to the main question „what works in social rehabilitation”, the attention of theoreticians and practitioners is directed to the evidence-based practice. Among numerous proposals and research data there is also Solution-Focused
Jana Chojecka
doaj +1 more source
, 2020 Based on an analysis of the ways in which constructionism is both implicitly and explicitly operational within contemporary higher arts education, this paper proposes a re-reading of the political stakes of constructionism.
Wilson, Mick,
core
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source