Results 101 to 110 of about 5,368,005 (298)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Mitochondrial Dynamics: In Cell Reprogramming as It Is in Cancer

Stem Cells International, 2017
Somatic cells can be reprogrammed into a pluripotent cellular state similar to that of embryonic stem cells. Given the significant physiological differences between the somatic and pluripotent cells, cell reprogramming is associated with a profound ...
Javier Prieto, Josema Torres
doaj   +1 more source

EVALUATION OF METHODS OF ANALYSIS TO DETERMINE THE SOMATIC CELL COUNT IN RAW MILK, KEPT IN THE COOLING TANK

Revista do Instituto de Latícinios Cândido Tostes, 2014
We analyzed the quality of raw milk from eight dairy property in Rio Grande do Norte, Brazil, stored in a cooling tank, in order to evaluate methods for determining somatic cell counts (SCC).
Manoel Pereira Neto, Adriano Henrique do Nascimento Rangel, Viviane Maia de Araújo, Dorgival Morais de Lima Júnior, Henrique Rocha de Medeiros, Luciano Patto Novaes   +5 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

ПРОУЧВАНЕ ВЛИЯНИЕТО НА НЯКОИ ФАКТОРИ ВЪРХУ БРОЯ НА СОМАТИЧНИТЕ КЛЕТКИ В МЛЯКОТО НА КРАВИ ОТ БЪЛГАРСКАТА ЧЕРНОШАРЕНА ПОРОДА

Животновъдни науки, 2011
The purpose of the current study was to determine the influence of different factors on the somatic cells in milk. The study comprised 1354 individual samples from Bulgarian Black and White cows from January 2009 to January 2010.
ТАТЯНА ИВАНОВА, МИЛЕН БОЙЧЕВ   +1 more
doaj  

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Asymmetric distribution of pl10 and bruno2, new members of a conserved core of early germline determinants in cephalochordates [PDF]

, 2016
SD is funded through a MASTS PhD studentship (St Andrews, UK). IMLS gratefully acknowledges previous funding from Marie Curie IEF postdoctoral fellowship, FP7 People Programme (Barcelona, Spain); as well as MASTS (Marine Alliance for Science and ...
Jordi Garcia-Fernàndez, Garcia-Fernandez, Jordi, Planas, Roser Febrero, Ildiko M. L. Somorjai, Simon C. Dailey, Ariadna Rossell Espier, Simon eDailey, Jordi eGarcia-Fernandez, Roser Febrero Planas, Roser eFebrero Planas, Ariadna eRossell Espier, Dailey, Simon Christopher, Somorjai, Ildiko Maureen Lara, Espier, Adriana Rossell   +13 more
core   +1 more source