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Cloning animals by somatic cell nuclear transfer – biological factors
Reproductive Biology and Endocrinology, 2003 Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced.
Enright Brian +3 more
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Factors influencing the somatic cell nuclear transfer efficiency in pigs [PDF]
Frontiers of Agricultural Science and Engineering, 2019 Using a data set from our laboratory, we assessed the effects of several factors on pig cloning efficiency. The results demonstrated that cells at high confluence (>90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall ...
Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI
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Applications of somatic cell nuclear transfer in goats
Journal of Advanced Veterinary and Animal Research, 2014 A number of animals with genetically identical appearance can be produced by somatic cell nuclear transfer (SCNT). From current advancement of SCNT and molecular techniques, production of a transgenic animal becomes easier. Although cloning efficiency in
Ton Yoisungnern, Ashit Kumar Paul
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Mitosis gives a brief window of opportunity for a change in gene transcription.
PLoS Biology, 2014 Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott +3 more
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浙江大学学报. 农业与生命科学版, 2018 Using gene editing technology based on CRISPR/Cas9n system and somatic cell nuclear transfer technology, we constructed a plasmid of two sgRNAs and Cas9 nickase, and green fluorescent protein (GFP) in the same vector followed by transient transfection of
WANG Shaohua +7 more
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Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos. [PDF]
PLoS ONE, 2011 Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.In this study, we analyzed the
Kai Wang +5 more
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BMC Genomics, 2018 Background Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries.
Yong Liu +13 more
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Generation of bovine transgenics using somatic cell nuclear transfer
Reproductive Biology and Endocrinology, 2003 The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have ...
Stice Steven L, Hodges Craig A
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Influences of somatic donor cell sex on and embryo development following somatic cell nuclear transfer in pigs [PDF]
Asian-Australasian Journal of Animal Sciences, 2017 Objective The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
Jae-Gyu Yoo +9 more
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Human Somatic Cell Nuclear Transfer Using Adult Cells [PDF]
Cell Stem Cell, 2014 Derivation of patient-specific human pluripotent stem cells via somatic cell nuclear transfer (SCNT) has the potential for applications in a range of therapeutic contexts. However, successful SCNT with human cells has proved challenging to achieve, and thus far has only been reported with fetal or infant somatic cells.
Chung, Young Gie +12 more
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