Results 31 to 40 of about 63,552 (337)

RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming. [PDF]

open access: yesPLoS ONE, 2016
The specific molecular mechanisms involved in somatic reprogramming remain unidentified. Removal of the oocyte genome is one of the primary causes of developmental failure in cloned embryos, whereas intact oocyte shows stronger reprogramming capability ...
Lin Bai   +6 more
doaj   +1 more source

Influences of somatic donor cell sex on and embryo development following somatic cell nuclear transfer in pigs [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2017
Objective The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
Jae-Gyu Yoo   +9 more
doaj   +1 more source

Mitosis gives a brief window of opportunity for a change in gene transcription.

open access: yesPLoS Biology, 2014
Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott   +3 more
doaj   +1 more source

Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing

open access: yesBMC Genomics, 2018
Background Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries.
Yong Liu   +13 more
doaj   +1 more source

Generation of bovine transgenics using somatic cell nuclear transfer

open access: yesReproductive Biology and Endocrinology, 2003
The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have ...
Stice Steven L, Hodges Craig A
doaj   +1 more source

Comparison of in vitro fertilization and nuclear transfer techniques in the production of buffalo pre-implantation embryos

open access: yesItalian Journal of Animal Science, 2010
This study was conducted to evaluate the timing of cleavage and blastocoel formation of in vitro fertilized and nuclear transfer embryos and to compare the efficiency of in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) techniques in ...
L.C. Cruz   +5 more
doaj   +1 more source

Somatic cell nuclear transfer: failures, successes and the challenges ahead.

open access: yesInternational Journal of Developmental Biology, 2019
Somatic cell nuclear transfer (SCNT) has a broad spectrum of potential applications, including rescue of endangered species, production of transgenic animals, drug production, and regenerative medicine.
M. Czernik   +4 more
semanticscholar   +1 more source

Biotechnological bases of the development of cloned pig embryos

open access: yesВавиловский журнал генетики и селекции, 2019
The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov   +2 more
doaj   +1 more source

Study on Embryo Transfer System for Production of Transgenic Pigs

open access: yesJournal of Animal Reproduction and Biotechnology, 2015
In the last 10 years, porcine somatic cell nuclear transfer to generate transgenic pig has been performed tremendous development with introduction and knockout of many genes.
Seungwon Na   +10 more
doaj   +1 more source

Epigenetic manipulation to improve mouse SCNT embryonic development

open access: yesFrontiers in Genetics, 2022
Cloned mammals can be achieved through somatic cell nuclear transfer (SCNT), which involves reprogramming of differentiated somatic cells into a totipotent state. However, low cloning efficiency hampers its application severely.
Yamei Li   +5 more
doaj   +1 more source

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