Results 31 to 40 of about 63,552 (337)
RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming. [PDF]
The specific molecular mechanisms involved in somatic reprogramming remain unidentified. Removal of the oocyte genome is one of the primary causes of developmental failure in cloned embryos, whereas intact oocyte shows stronger reprogramming capability ...
Lin Bai+6 more
doaj +1 more source
Influences of somatic donor cell sex on and embryo development following somatic cell nuclear transfer in pigs [PDF]
Objective The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
Jae-Gyu Yoo+9 more
doaj +1 more source
Mitosis gives a brief window of opportunity for a change in gene transcription.
Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott+3 more
doaj +1 more source
Background Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries.
Yong Liu+13 more
doaj +1 more source
Generation of bovine transgenics using somatic cell nuclear transfer
The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have ...
Stice Steven L, Hodges Craig A
doaj +1 more source
This study was conducted to evaluate the timing of cleavage and blastocoel formation of in vitro fertilized and nuclear transfer embryos and to compare the efficiency of in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) techniques in ...
L.C. Cruz+5 more
doaj +1 more source
Somatic cell nuclear transfer: failures, successes and the challenges ahead.
Somatic cell nuclear transfer (SCNT) has a broad spectrum of potential applications, including rescue of endangered species, production of transgenic animals, drug production, and regenerative medicine.
M. Czernik+4 more
semanticscholar +1 more source
Biotechnological bases of the development of cloned pig embryos
The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov+2 more
doaj +1 more source
Study on Embryo Transfer System for Production of Transgenic Pigs
In the last 10 years, porcine somatic cell nuclear transfer to generate transgenic pig has been performed tremendous development with introduction and knockout of many genes.
Seungwon Na+10 more
doaj +1 more source
Epigenetic manipulation to improve mouse SCNT embryonic development
Cloned mammals can be achieved through somatic cell nuclear transfer (SCNT), which involves reprogramming of differentiated somatic cells into a totipotent state. However, low cloning efficiency hampers its application severely.
Yamei Li+5 more
doaj +1 more source