Results 91 to 100 of about 555,969 (265)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analytical solutions for performance of existing shield tunnel subjected to overlying excavation under anti-uplift portal frame

Yantu gongcheng xuebao
The heave of the existing shield tunnels will be induced due to the above excavation, and then structural damages, including leakage and cracking, easily happen.
LIU Yuan 1, 2, 3, CHEN Renpeng 1, 2, 3, CHENG Hongzhan 1, 2, 3, WU Huaina 1, 2, 3, ZHANG Kai 1, 2, 3
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Deep learning-based prediction method for chamber pressure field in shield machines

Yantu gongcheng xuebao
The chamber pressure is a key factor in the core issues of the stress state of equipment and the stability of tunnel face during shield tunneling. It exhibits significant spatial variability, and its formation and evolution originate from the complex ...
ZHANG Chao 1, 2, 3, ZHU Minxiang 1, LANG Zhixiong 1, CHEN Renpeng 1, 2, 3, , , CHENG Hongzhan 1, 2, 3
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Experimental study on the fracture surface morphological characteristics and permeability characteristics of sandstones with different particle sizes

Energy Science & Engineering
To study the seepage law and sectional morphological characteristics in splitting sandstones of different grain sizes, three‐dimensional morphological scanning tests and seepage characteristics tests are conducted on the splitting rock samples of ...
Cao Zhengzheng, Yang Xiangqian, Zhang Peiding, Li Zhenhua, Du Feng, Wang Wenqiang, Chen Huanqi   +6 more
doaj   +1 more source

Centrifuge modeling of excavation and numerical analyses of soil arching below excavation base

Yantu gongcheng xuebao
Soil arching may be generated below excavation base. This kind of soil arching is featured with different boundary conditions of mechanism and displacement compared with that above trapdoor, of which the development orientation is the same as that of ...
WU Kai 1, 2, 3, CHEN Renpeng 1, 2, 3, MENG Fanyan 1, 2, 3, WANG Hanlin 1, 2, 3, CHENG Hongzhan 1, 2, 3
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Experimental study on mechanical behavior of sand and silty clay under vertical unloading

Yantu gongcheng xuebao
The ultimate unloading ratio and the critical unloading ratio are the crucial parameters for determining the depth of disturbance below excavation base.
WU Kai 1, 2, 3, CHEN Renpeng 1, 2, 3, MENG Fanyan 1, 2, 3, HU Bo 2
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source