Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Spatial distribution of entomopathogenic nematodes and their relationship with soil chemical attributes in tropical agroecosystems. [PDF]
Souza N +5 more
europepmc +1 more source
USING OPTIMAL CONTROL TO CHARACTERIZE THE ECONOMIC AND ECOLOGICAL IMPLICATIONS OF SPATIAL EXTERNALITIES [PDF]
This study uses optimal control theory to examine the dynamic features of agricultural landscapes characterized by spatial externalites. A stylized system of agricultural production and groundwater flow is used to illustrate how spatial externalities ...
Marshall, Elizabeth P. +1 more
core
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Rural revitalization, carbon emissions, and infectious disease transmission in China: nonlinear dynamics, spatial spillovers, and moderating effect. [PDF]
Shang Q, Zheng X, Wang Y.
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Spatiotemporal patterns of zoonotic cutaneous leishmaniasis in the high-burden provinces of Morocco, 1995-2022: an exploratory analysis of environmental and socio-demographic correlates. [PDF]
Harkat C +6 more
europepmc +1 more source
Cell surface CD11c as a neutrophil aging marker molecule
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki +5 more
wiley +1 more source
Epidemiologically validated spatial modelling reveals fine-scale heat health risks. [PDF]
Su Z +15 more
europepmc +1 more source

