Results 71 to 80 of about 11,516,984 (249)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Diallel analysis and genetic differentiation of tropical and temperate maize inbred lines

Crop Breeding and Applied Biotechnology, 2018
This study aimed to evaluate hybrids obtained by the cross among temperate × tropical elite maize lines, and to determine the genetic diversity and population differentiation of these inbred lines.
Vania Portes Kulka, Tereza Aparecida da Silva, Rodrigo I. Contreras-Soto, Carlos Maldonado, Freddy Mora, Carlos Alberto Scapim   +5 more
doaj  

Combining ability of wheat parents in two generations

Semina: Ciências Agrárias, 2013
The objective of this study were to estimate the general ability and specific combining ability in ten wheat genotypes in two generations (F1 and F2) to indicate parents and hybrid combinations that are promising to achieve new favorable combinations ...
Elesandro Bornhofen, Giovani Benin , Gilvani Matei, Cristiano Lemes da Silva, Eduardo Beche, Eduardo Stefani Pagliosa , Thaís Raquel Hagemann , Cilas Pinnow   +7 more
doaj   +1 more source

Combining ability analysis for yield and yield components in bold grained rice (Oryza sativa L.) of Assam [PDF]

, 2009
Combining ability analysis for grain yield and its components was carried out in eight parental diallel crosses of bold grained rice excluding reciprocals.
R Chakraborty, Paul, S. B., Dutta, B. K., S.B. Paul, Chakraborty, Supriyo, B.K. Dutta, Supriyo Chakraborty, Chakraborty, R.   +7 more
core  

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Assessment of gene action and combining ability for fibre and yield contributing trais in interspecific and intraspecific hybrids of cotton

Czech Journal of Genetics and Plant Breeding, 2018
Combining ability analysis was performed in order to identify high-yielding genotypes and hybrids. Six lines were crossed with three testers using a line × tester mating design. General combining ability (GCA) of parents, specific combining ability (SCA)
Sana MUNIR, Muhammad Kamran QURESHI, Ahmad Naeem SHAHZAD, Hamid MANZOOR, Muhammad Ahmad SHAHZAD, Kashif ASLAM, Habib-Ur-Rehman ATHAR   +6 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Elucidating the genetic potential of hybrids derived from elite maize (Zea mays L.) inbreds for economic traits under North-Western Himalayan condition

Electronic Journal of Plant Breeding
This study was conducted to evaluate the combining ability of newly acquired maize inbred lines over the environments. Twenty-eight crosses developed by crossing eight elite inbreds in a half-diallel mating design were evaluated during kharif, 2019 for ...
Gaurav Sharma*, Uttam Chandel, Sawan Kumar, Satish Kumar Guleria and Nimit Kumar
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source