Results 31 to 40 of about 6,875,638 (265)

Analysis of liposome model systems by time-of-flight secondary ion mass spectrometry [PDF]

, 2014
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is an important technique for studying chemical composition of micrometer scale objects because of its high spatial resolution imaging capabilities and chemical specificity. In this work, we focus
Per Malmberg, Ewing, Andrew G.,, Andrew G. Ewing, Angerer, Tina B.,, Fletcher, John S., Jacqueline D. Keighron, Lovric, Jelena,, Ewing, Andrew G, Lovric, Jelena, Fletcher, John S.,, Angerer, Tina B., Keighron, Jacqueline, Xianchan Li, John S. Fletcher, Li, Xianchan,, Ewing, Andrew G,, Malmberg, Per,, Keighron, Jacqueline D.,, Jelena Lovrić, Fletcher, John, Li, Xianchan, Keighron, Jacqueline,, Tina B. Angerer, Malmberg, Per, Ewing, Andrew, Xianchan, Li   +25 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging

Journal of Lipid Research, 2014
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and the early embryo development stages with ...
Hua Tian, John S. Fletcher, Raphael Thuret, Alex Henderson, Nancy Papalopulu, John C. Vickerman, Nicholas P. Lockyer   +6 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Absorption mode FTICR mass spectrometry imaging [PDF]

, 2013
Fourier transform ion cyclotron resonance mass spectrometry offers the highest mass resolving power for molecular imaging experiments. This high mass resolving power ensures that closely spaced peaks at the same nominal mass are resolved for proper image
Konijnenburg, Marco, Heeren, Ron M. A., O’Connor, Peter B., Heeren, R.M.A., Smith, Donald Francis, Kilgour, David P. A., Kilgour, D.P.A., Konijnenburg, M., Smith, D.F., Sub Biomol.Mass Spectrometry & Proteom., O'Connor, P.B., Biomolecular Mass Spectrometry and Proteomics   +11 more
core   +1 more source

A Review of ex vivo Elemental Mapping Methods to Directly Image Changes in the Homeostasis of Diffusible Ions (Na+, K+, Mg2 +, Ca2 +, Cl–) Within Brain Tissue

Frontiers in Neuroscience, 2020
Diffusible ions (Na+, K+, Mg2+, Ca2+, Cl–) are vital for healthy function of all cells, especially brain cells. Unfortunately, the diffusible nature of these ions renders them difficult to study with traditional microscopy in situ within ex vivo brain ...
David Hartnell, David Hartnell, David Hartnell, Wendy Andrews, Wendy Andrews, Wendy Andrews, Nicole Smith, Haibo Jiang, Erin McAllum, Ramesh Rajan, Frederick Colbourne, Frederick Colbourne, Melinda Fitzgerald, Melinda Fitzgerald, Melinda Fitzgerald, Virginie Lam, Virginie Lam, Ryusuke Takechi, Ryusuke Takechi, M. Jake Pushie, Michael E. Kelly, Mark J. Hackett, Mark J. Hackett, Mark J. Hackett   +23 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Gold and silver nanoparticle-assisted laser desorption ionization mass spectrometry compatible with secondary ion mass spectrometry for lipid analysis

, 2014
To provide a convenient organic matrix-free spray-on sample preparation for laser desorption ionization (LDI) that is compatible with secondary ion mass spectrometry (SIMS) imaging mass spectrometry (IMS) techniques, silver and gold nanoparticles (NPs ...
Per Malmberg, Amir Saeid Mohammadi, Andrew G. Ewing, Mohammadi, Amir Saeid, Fletcher, John,, Fletcher, John S., Fletcher, John, Ewing, Andrew G, Mohammadi, Amir Saeid,, Fletcher, John S.,, Malmberg, Per, John S. Fletcher, Ewing, Andrew, Ewing, Andrew G,, Ewing, Andrew,, Malmberg, Per,   +15 more
core   +1 more source

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Frontiers in Chemistry, 2022
Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of ...
Xiaoping Zhu, Xiaoping Zhu, Tianyi Xu, Tianyi Xu, Chen Peng, Shihua Wu, Shihua Wu   +6 more
doaj   +1 more source