Results 71 to 80 of about 3,143,049 (329)
Development of visible light‐sensitive human neuropsin (OPN5) via single amino acid substitution
FEBS Letters, EarlyView.The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Yusuke Sakai +2 more
wiley +1 more source
Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR
Nature Communications, 2018 The β-barrel assembly machinery (BAM) catalyzes β-barrel protein insertion into the outer membrane of E.coli. Here authors employ high-sensitivity solid-state NMR to reveal how the lipid environment and formation of the BamA-BamCDE complex affect BamA ...
Cecilia Pinto +5 more
doaj +1 more source
Nature Communications, 2023 The authors realize longitudinal deep-brain imaging through an intact mouse skull by constructing a high-speed reflection matrix microscope at 1.3 µm wavelength and developing a computational conjugate adaptive optics algorithm eliminating skull ...
Yongwoo Kwon +7 more
doaj +1 more source
FEBS Letters, EarlyView.C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi +5 more
wiley +1 more source
Scientific ReportsVersatile methods for the quantification of intracellular cholesterol are essential for understanding cellular physiology and for diagnosing disorders linked to cholesterol metabolism.
Enrico Baria +8 more
doaj +1 more source
Nature Communications, 2022 By using laser tweezers, the authors show that a single α-catenin molecule does not resist force on F-actin. However, clustering of multiple molecules and force applied toward F-actin pointed end engage a molecular switch in α-catenin, which unfolds and ...
C. Arbore +8 more
doaj +1 more source
FEBS Letters, EarlyView.Spinal muscular atrophy (SMA) is a genetic disease affecting motor neurons. Individuals with SMA experience mitochondrial dysfunction and oxidative stress. The aim of the study was to investigate the effect of an antioxidant and neuroprotective substance, ergothioneine (ERGO), on an SMNΔ7 mouse model of SMA.
Francesca Cadile +8 more
wiley +1 more source
Mid-infrared single-photon 3D imaging
Light: Science & Applications, 2023 Active mid-infrared (MIR) imagers capable of retrieving three-dimensional (3D) structure and reflectivity information are highly attractive in a wide range of biomedical and industrial applications.
Jianan Fang +4 more
doaj +1 more source
Detection of Single Ion Spectra by Coulomb Crystal Heating
, 2010 The coupled motion of ions in a radiofrequency trap has been used to connect the frequency- dependent laser-induced heating of a sympathetically cooled spectroscopy ion with changes in the fluorescence of a laser-cooled control ion.
C. Ricardo Viteri +6 more
core +1 more source
Structural dynamics of the plant hormone receptor ETR1 in a native‐like membrane environment
FEBS Letters, EarlyView.The present study unveils the structural and signaling dynamics of ETR1, a key plant ethylene receptor. Using an optimized nanodisc system and solution NMR, we captured full‐length ETR1 in a native‐like membrane environment. Our findings reveal dynamic domain uncoupling and Cu(I)‐induced rigidification, providing the first evidence of metal‐triggered ...
Moritz Lemke +2 more
wiley +1 more source