Results 31 to 40 of about 62,614 (258)

Assessment of the effectiveness of methods for the selection of sperm with increased DNA fragmentation in ART cycles [PDF]

BIO Web of Conferences
Sperm DNA stability is significant in male infertility and favorable reproductive outcomes. Increased DNA fragmentation in spermatozoa can negatively affect the fertilization potential and the kinetics of embryo development.
Leontyeva Eugene, Kim Alena, Zastavskiy Ivan, Yakhyarova Mukhabbat, Polumiskov Vadim   +4 more
doaj   +1 more source

Measuring Pre- and Post-Copulatory Sexual Selection and Their Interaction in Socially Monogamous Species with Extra-Pair Paternity

Cells, 2021
When females copulate with multiple males, pre- and post-copulatory sexual selection may interact synergistically or in opposition. Studying this interaction in wild populations is complex and potentially biased, because copulation and fertilization ...
Emily Rebecca Alison Cramer
doaj   +1 more source

Modeling diffusive search by non-adaptive sperm: Empirical and computational insights.

PLoS Computational Biology
During fertilization, mammalian sperm undergo a winnowing selection process that reduces the candidate pool of potential fertilizers from ~106-1011 cells to 101-102 cells (depending on the species).
Benjamin M Brisard, Kylie D Cashwell, Stephanie M Stewart, Logan M Harrison, Aidan C Charles, Chelsea V Dennis, Ivie R Henslee, Ethan L Carrow, Heather A Belcher, Debajit Bhowmick, Paul W Vos, Maciej Majka, Martin Bier, David M Hart, Cameron A Schmidt   +14 more
doaj   +1 more source

ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function

FEBS Open Bio, EarlyView.
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley, Luke W. Molesworth, Nethma H. Waduge, Fahimeh Eskandari, Peter R. Boag, Gregory M. Davis   +5 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A PMMA-Based Microfluidic Device for Human Sperm Evaluation and Screening on Swimming Capability and Swimming Persistence

Micromachines, 2020
The selection of high-quality sperm is essential to the success of in vitro fertilization (IVF). As human cervical mucus has a high viscosity, without enough swimming persistence, human sperm clouds cannot arrive at the ampulla to fertilize the egg.
Yimo Yan, Haoran Liu, Boxuan Zhang, Ran Liu   +3 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Sperm selection before intracytoplasmic sperm injection

Current Trends in Clinical Embriology, 2015
In assisted reproduction techniques (ART), the success of embryo development depends mostly on the quality of gametes, and pregnancy outcome is strictly related to both the oocyte and the fertilizing sperm quality. Therefore, during ART, it is mandatory to select a mature and viable sperm with high DNA integrity. Routine sperm preparation and selection
TALEVI, RICCARDO, GUALTIERI, ROBERTO
openaire   +2 more sources

Intelligent Micro/Nanorobots for Targeted Interventional Therapy: From Bench to Clinic

Advanced Robotics Research, EarlyView.
Zirui Zhang et al. reviewed the application and challenges of mobile nanomachines in interventional therapy. By converting exogenous energy, including chemical, magnetic, optical, and ultrasonic sources, into mechanical forces, micro/nanorobots (MNRs) enable precise actuation at unprecedented scales. Evolving far beyond traditional drug delivery, these
Zirui Zhang, Yu Dong, Yongsheng Du, Rui Gao, Lin Zhang, Fei Tong   +5 more
wiley   +1 more source

Live Births Following Zona Pellucida–Binding Sperm Selection in Recurrent Implantation Failure: Two Case Reports

Fertility & Reproduction
Recurrent implantation failure (RIF) poses a significant challenge in assisted reproductive technologies (ART). Intracytoplasmic sperm injection (ICSI), while effective for male factor infertility, bypasses natural sperm selection, potentially reducing ...
Mahin Izadi, Serajoddin Vahidi Mehrjardi, Mohammad Ali Khalili, Mohammad Ebrahim Rezvani, Behrouz Aflatoonian   +4 more
doaj   +1 more source