Advanced Intelligent Discovery, EarlyView.Sequential multicolor fluorescence imaging in dynamic microsystems is constrained by acquisition speed and excitation dose. This study introduces a real‐time framework to reconstruct spectrally separated channels from reduced cross‐channel acquisitions (frames containing mixed spectral contributions).
Juan J. Huaroto +3 more
wiley +1 more source
Geometric Nonuniformity Localizes Valence Orbitals in Gold Clusters: Case Study on [Au23(SC6H11)16]–
Angewandte Chemie, EarlyView.The electronic structure of [Au23(SC6H11)16]–, a monolayer‐protected gold cluster with a nonuniform Au13 core, was investigated. Anion photoelectron spectroscopy and density functional theory calculations revealed that the highest occupied molecular orbital (HOMO) and HOMO‐1 are localized on the two triangular Au3 units at the ends of the Au13 core ...
Yosuke Asami +3 more
wiley +2 more sources
HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle Genes. [PDF]
CellsCheng S +4 more
europepmc +1 more source
Cell Segmentation Beyond 2D—A Review of the State‐of‐the‐Art
Advanced Intelligent Discovery, EarlyView.Cell segmentation underpins many biological image analysis tasks, yet most deep learning methods remain limited to 2D despite the inherently 3D nature of cellular processes. This review surveys segmentation approaches beyond 2D, comparing 2.5D and fully 3D methods, analyzing 31 models and 32 volumetric datasets, and introducing a unified reference ...
Fabian Schmeisser +6 more
wiley +1 more source
CSAKD: Determining Absolute Ligand Affinities From 19F NMR Chemical Shift Anisotropy
Angewandte Chemie, EarlyView.Affinity determination is crucial in drug discovery, yet remains difficult for weakly binding fragments. We introduce chemical shift anisotropy KD$K_{\text{D}}$ (CSAKD) by 19F$^{19}{\rm F}$ NMR relaxation experiments, a titration‐free method that requires no isotopic labeling.
Simon H. Rüdisser +2 more
wiley +2 more sources
Mesoscopic Fluorescence Imaging of Light-Triggered Chemotherapeutic Release in Cancer Spheroid Models. [PDF]
PharmaceuticsKluiszo E +7 more
europepmc +1 more source
Stable Neural Signal Recording Processed by Memristor‐Based Reservoir Computing System
Advanced Intelligent Systems, EarlyView.This work introduces a memristor‐based reservoir computing (RC) system for real‐time, energy‐efficient processing of neural signals in brain‐machine interface (BMI). Combined with flexible mesh neural probes with tissue‐like flexibility and subcellular‐scale features that enable consistent, long‐term tracking of single‐cell neural activities, the ...
Soohyeon Kim +10 more
wiley +1 more source
Mitochondria‐Targeting Moieties Based on N‐Tethered Pyridinium Cations
Angewandte Chemie, EarlyView.Pyridinium cations were benchmarked as mitochondria‐targeting moieties in a panel of N‐tethered fluorescent‐, bioactive‐, and inert‐cargo conjugates. 3,5‐Diphenylpyridinium (DPPy+) is a competent triphenylphosphonium (TPP+) surrogate, combining high mitochondria‐targeting efficiency with lower intrinsic effects on mitochondrial function.
Ivan Džajić +27 more
wiley +2 more sources
Distinct migration patterns of adult neural stem cells derived from hippocampal and ventricular niches. [PDF]
Front Aging NeurosciAmartumur S +4 more
europepmc +1 more source
Spatial Cell Death and Oxidative Stress Dynamics in Gas Plasma‐Treated Tumor Tissues
Advanced NanoBiomed Research, EarlyView.Schematic representation of the four experimental models to study tissue penetration and oxidation. Four tissue models were used. Human pancreatic cancer cells were grown on the chorioallantois membrane of chicken embryos and gas plasma‐treated in ovo, murine colorectal tumor tissue was gas plasma‐exposed ex vivo, murine squamous cell carcinoma cells ...
Anke Schmidt +4 more
wiley +1 more source