Results 1 to 10 of about 79,551 (308)

Quality evaluation of cell spheroids for transplantation by monitoring oxygen consumption using an on-chip electrochemical device

Biotechnology Reports, 2022
Three-dimensional cell spheroids are superior cell-administration form for cell-based therapy which generally exhibit superior functionality and long-term survival after transplantation.
Mari Tsujimura, Kosuke Kusamori, Kodai Takamura, Temmei Ito, Takatoshi Kaya, Kazunori Shimizu, Satoshi Konishi, Makiya Nishikawa   +7 more
doaj   +1 more source

Electron microscopy imaging and mechanical characterization of T47D multicellular tumor spheroids-Older spheroids reduce interstitial space and become stiffer.

PLoS ONE, 2023
Multicellular cancer spheroids are an in vitro tissue model that mimics the three-dimensional microenvironment. As spheroids grow, they develop the gradients of oxygen, nutrients, and catabolites, affecting crucial tumor characteristics such as ...
Mathangi Madhavan, Devina Jaiswal, Sarah Karlberg, Alexis Duggan, Hassan A Almarshad, Kevin P Claffey, Kazunori Hoshino   +6 more
doaj   +2 more sources

Characterization of 3D heterocellular spheroids of pancreatic ductal adenocarcinoma for the study of cell interactions in the tumor immune microenvironment

Frontiers in Oncology, 2023
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignancies nowadays. The available chemo- and immunotherapies are often ineffective in treating PDAC due to its immunosuppressive and highly desmoplastic tumor immune microenvironment ...
Giulio Giustarini, Germaine Teng, Andrea Pavesi, Andrea Pavesi, Giulia Adriani, Giulia Adriani   +5 more
doaj   +1 more source

Spontaneous spheroids from alveolar bone-derived mesenchymal stromal cells maintain pluripotency of stem cells by regulating hypoxia-inducible factors

Biological Research, 2023
Background Spontaneous spheroid culture is a novel three-dimensional (3D) culture strategy for the rapid and efficient selection of progenitor cells.
Ni Li, Xiaofeng Dai, Fei Yang, Yang Sun, Xingwen Wu, Qianrong Zhou, Kai Chen, Jian Sun, Wei Bi, Le Shi, Youcheng Yu   +10 more
doaj   +1 more source

Characterization of Three-Dimensional Trophoblast Spheroids: An Alternative Model to Study the Physiological Properties of the Placental Unit

Cells, 2022
It was postulated that 3D cell culture models more accurately reflect the complex tissue physiology and morphology in comparison to 2D cell monolayers.
Violeta Stojanovska, Susanne Arnold, Mario Bauer, Hermann Voss, Stefan Fest, Ana Claudia Zenclussen   +5 more
doaj   +1 more source

Co-cultured spheroids of human periodontal ligament mesenchymal stem cells and vascular endothelial cells enhance periodontal tissue regeneration

Regenerative Therapy, 2020
Introduction: Human periodontal ligament mesenchymal stem cells (hPDLMSCs) have been known that they play important roles in homeostasis and regeneration of periodontal tissues. Additionally, spheroids are superior to monolayer-cultured cells.
Kotaro Sano, Michihiko Usui, Yuki Moritani, Kohji Nakazawa, Tomoya Hanatani, Hisataka Kondo, Mitsushiro Nakatomi, Satoru Onizuka, Takanori Iwata, Tsuyoshi Sato, Akifumi Togari, Wataru Ariyoshi, Tatsuji Nishihara, Keisuke Nakashima   +13 more
doaj   +1 more source

Stem cell spheroids production for wound healing with a reversible porous hydrogel

Materials Today Advances, 2022
Adipose derived stem cell (ASC) spheroids show enhanced cellular viability and paracrine function to promote tissue regeneration and cell therapy effect.
Jiujiang Zeng, Xi Chen, Jiahui Zhang, Yechi Qin, Kunxi Zhang, Xinping Li, Haiyan Cui   +6 more
doaj   +1 more source

Functional 3D human primary hepatocyte spheroids made by co-culturing hepatocytes from partial hepatectomy specimens and human adipose-derived stem cells.

PLoS ONE, 2012
We have generated human hepatocyte spheroids with uniform size and shape by co-culturing 1∶1 mixtures of primary human hepatocytes (hHeps) from partial hepatectomy specimens and human adipose-derived stem cells (hADSCs) in concave microwells.
Da Yoon No, Seung-A Lee, Yoon Young Choi, DoYeun Park, Ju Yun Jang, Dong-Sik Kim, Sang-Hoon Lee   +6 more
doaj   +1 more source

One-step visualization of natural cell activities in non-labeled living spheroids

Scientific Reports, 2022
3D cultured cell aggregates, including spheroids, reflect the gene expression patterns of living tissues/organs. Mass preparation of spheroids enables high-throughput drug screening (HTS).
Shotaro Tanaka, Kotaro Takizawa, Fumio Nakamura   +2 more
doaj   +1 more source

Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity

iScience, 2023
Summary: Dynamic changes in cell properties lead to intratumor heterogeneity; however, the mechanisms of nongenetic cellular plasticity remain elusive.
Roberto Coppo, Jumpei Kondo, Keita Iida, Mariko Okada, Kunishige Onuma, Yoshihisa Tanaka, Mayumi Kamada, Masayuki Ohue, Kenji Kawada, Kazutaka Obama, Masahiro Inoue   +10 more
doaj   +1 more source