Results 21 to 30 of about 79,551 (308)

Preparation of Spheroids from Primary Pig Cells in a Mid-Scale Bioreactor Retaining Their Myogenic Potential

Cells, 2022
Three-dimensional cell culture techniques mimic the in vivo cell environment more adequately than flat surfaces. Spheroids are multicellular aggregates and we aimed to produce scaffold-free spheroids of myogenic origin, called myospheres, using a mid ...
Katja Stange, Amir Keric, Andreas Friese, Monika Röntgen   +3 more
doaj   +1 more source

3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture

Journal of Biological Engineering, 2019
Background Three dimensional (3D) cell cultures have been an area of increasing interest and relevance across several research fields including drug discovery, developmental biology and stem cell-based therapies.
Mo Chen, Marie-Laure Vial, James A. St John   +2 more
doaj   +1 more source

Cryopreservation method for spheroids and fabrication of scaffold-free tubular constructs.

PLoS ONE, 2020
Cryopreservation is a method used for preserving living cells by cooling them to very low temperatures. Although cryopreservation methods for oocytes and embryos have been developed for use in reproductive medicine, there are no established methods yet ...
Kenichi Arai, Daiki Murata, Shoko Takao, Ana Raquel Verissiomo, Koichi Nakayama   +4 more
doaj   +1 more source

Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants [PDF]

, 2023
Spheroids are a powerful tool for basic research and to reduce or replace in vivo (animal) studies but are not routinely banked nor shared. Here, we report the successful cryopreservation of hepatocyte spheroids using macromolecular (polyampholyte ...
Yanan Gao, Bissoyi, Akalabya; id_orcid, Gibson, Matthew I.; id_orcid, Yanan Gao (360685), Matthew I. Gibson, Matthew I. Gibson (1273788), Qiongyu Guo, Tomás, Ruben, M. F., Ruben M. F. Tomás, Akalabya Bissoyi (13256682), Akalabya Bissoyi, Bissoyi, Akalabya, Guo, Qiongyu, Gibson, Matthew I., Ruben M. F. Tomás (6910730), Gao, Yanan, Qiongyu Guo (757990), Tomás, Ruben M. F.   +17 more
core   +1 more source

Fast generation of forebrain oligodendrocyte spheroids from human embryonic stem cells by transcription factors

iScience, 2022
Summary: Oligodendrocyte spheroids (OL-spheroids) containing oligodendrocytes and neurons provide an accessible system to dissect demyelinating diseases and test therapeutic treatment.
Ling Ma, Yuting Mei, Peibo Xu, Yan Cheng, Zhiwen You, Xiaoli Ji, Deyi Zhuang, Wenhao Zhou, Yuejun Chen, Man Xiong   +9 more
doaj   +1 more source

Histological analysis of spheroids.

, 2020
Non-cryopreserved spheroids (A), cryopreserved spheroids using PBS solution (B), and cryopreserved spheroids using StemSure® solution (C) were analyzed by hematoxylin and eosin staining.
Kenichi Arai (6090824), Daiki Murata (5059025), Shoko Takao (8661690), Ana Raquel Verissiomo (8661693), Koichi Nakayama (790706)   +4 more
core   +1 more source

Monolayer to MTS : using SEM, HIM, TEM and SERS to compare morphology, nanosensor uptake and redox potential in MCF7 cells [PDF]

, 2015
This research received support from the QNano Project http://www.qnano-ri.eu which is financed by the European Community Research Infrastructures under the FP7 Capacities Programme (Grant No.
Campbell, C.J., C. J. Campbell, Bell, A.P., A. P. Bell, Jamieson, L.E., Harrison, D.J., L. E. Jamieson, D. J. Harrison, Harrison, D.J.; id_orcid   +8 more
core   +1 more source

ASC Spheroid Geometry and Culture Oxygenation Differentially Impact Induction of Preangiogenic Behaviors in Endothelial Cells

Cell Transplantation, 2015
Cell-based angiogenic therapies offer potential for the repair of ischemic injuries, while avoiding several of the limitations associated with material-based growth factor delivery strategies. Evidence supports that applying MSCs as spheroids rather than
Matthew L. Skiles, Brandon Hanna, Lindsay Rucker, Allison Tipton, Aidan Brougham-Cook, Ehsan Jabbarzadeh, James O. Blanchette   +6 more
doaj   +1 more source

Temperature of the spheroidal state [PDF]

Science, 1884
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openaire   +4 more sources

Cell Line-Dependent Effects of Spheroid Formation Method on Drug Response in Melanoma Models

Cells
In this study, we evaluated the impact of different in vitro 3D culture modelling methods on the activity of doxorubicin (DOX) and 5-fluorouracil (5-FU) in human melanoma spheroids. Human melanoma A375 and IGR39 spheroids were generated using the hanging
Akvilė Žilytė, Vilma Petrikaitė
doaj   +1 more source