Results 11 to 20 of about 47,685 (161)

Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label. [PDF]

open access: yesAppl Magn Reson, 2021
AbstractStructural investigations of proteins and their biological complexes are now frequently complemented by distance constraints between spin labeled cysteines generated using double electron–electron resonance (DEER) spectroscopy, via site directed spin labeling (SDSL).
Heaven G   +3 more
europepmc   +5 more sources

Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy [PDF]

open access: yesMolecules, 2022
We present a new photoswitchable spin label for light-induced pulsed electron paramagnetic resonance dipolar spectroscopy (LiPDS), the photoexcited triplet state of erythrosin B (EB), which is ideal for biological applications.
Arnau Bertran   +7 more
doaj   +2 more sources

Rigid and stable nitroxide spin label for high-resolution distance measurements on proteins by DEER experiments [PDF]

open access: yesMagnetic Resonance Letters
The distance distributions between two site-specifically anchored spin labels in a protein, measured by pulsed electron-electron double resonance (PELDOR or DEER), provide rich sources of structural and conformational restraints on the proteins or their ...
Ya-Ting Chen   +6 more
doaj   +2 more sources

RosettaEPR: rotamer library for spin label structure and dynamics. [PDF]

open access: yesPLoS ONE, 2013
An increasingly used parameter in structural biology is the measurement of distances between spin labels bound to a protein. One limitation to these measurements is the unknown position of the spin label relative to the protein backbone. To overcome this
Nathan S Alexander   +5 more
doaj   +2 more sources

Exploring the Interactions of Ruthenium (II) Carbosilane Metallodendrimers and Precursors with Model Cell Membranes through a Dual Spin-Label Spin-Probe Technique Using EPR [PDF]

open access: yesBiomolecules, 2019
Dendrimers exhibit unique interactions with cell membranes, arising from their nanometric size and high surface area. To a great extent, these interactions define their biological activity and can be reported in situ by spin-labelling techniques.
Riccardo Carloni   +8 more
doaj   +2 more sources

Resting state BOLD-perfusion coupling patterns using multiband multi-echo pseudo-continuous arterial spin label imaging [PDF]

open access: yesScientific Reports
The alteration of neurovascular coupling (NVC), where acute localized blood flow increases following neural activity, plays a key role in several neurovascular processes including aging and neurodegeneration.
Alexander D. Cohen   +4 more
doaj   +2 more sources

Spin-Lattice Relaxation Rates of Lipid Spin Labels as a Measure of Their Rotational Diffusion Rates in Lipid Bilayer Membranes

open access: yesMembranes, 2022
The spin-lattice relaxation rate (T1−1) of lipid spin labels obtained from saturation recovery EPR measurements in deoxygenated membranes depends primarily on the rate of the rotational diffusion of the nitroxide moiety within the lipid bilayer.
Witold K. Subczynski, Justyna Widomska
doaj   +1 more source

Combination of fluorescent and spin labels: a powerful method for the optimization of hydrophilic membranes for the separation of oil-in-water emulsions

open access: yesChimica Techno Acta, 2023
A new method for assessing the quality of fibre coating based on a combination of fluorescence microscopy and electron paramagnetic resonance is presented in this work.
Rita S. Alqubelat   +3 more
doaj   +1 more source

Spin-labeled lipid A [PDF]

open access: yesAnalytical Biochemistry, 2008
Lipopolysaccharide (LPS), a major component of the outer membranes of gram-negative bacteria, is composed of a polysaccharide chain attached to a lipid A base that contains a disaccharide headgroup with two negative phosphate groups and at least four acyl chains.
Lynn E, Bretscher   +2 more
openaire   +2 more sources

Librational Dynamics of Spin-Labeled Membranes at Cryogenic Temperatures From Echo-Detected ED-EPR Spectra

open access: yesFrontiers in Molecular Biosciences, 2022
Methods of electron spin echo of pulse electron paramagnetic resonance (EPR) spectroscopy are increasingly employed to investigate biophysical properties of nitroxide-labeled biosystems at cryogenic temperatures.
Rosa Bartucci, Erika Aloi
doaj   +1 more source

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