PIASgamma is required for faithful chromosome segregation in human cells. [PDF]
PLoS ONE, 2006 BACKGROUND:The precision of the metaphase-anaphase transition ensures stable genetic inheritance. The spindle checkpoint blocks anaphase onset until the last chromosome biorients at metaphase plate, then the bonds between sister chromatids are removed ...
Laura A Díaz-Martínez +6 more
doaj +1 more source
A Mathematical Framework for Kinetochore-Driven Activation Feedback in the Mitotic Checkpoint [PDF]
, 2017 Proliferating cells properly divide into their daughter cells through a process that is mediated by kinetochores, protein-complexes that assemble at the centromere of each sister chromatid. Each kinetochore has to establish a tight bipolar attachment to the spindle apparatus before sister-chromatid separation is initiated.
arxiv +1 more source
Monitoring spindle orientation: Spindle position checkpoint in charge
Cell Division, 2010 Every cell division in budding yeast is inherently asymmetric and counts on the correct positioning of the mitotic spindle along the mother-daughter polarity axis for faithful chromosome segregation.
Pereira Gislene +2 more
doaj +1 more source
Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint. [PDF]
PLoS ONE, 2016 The multi-functional molecular motor cytoplasmic dynein performs diverse essential roles during mitosis. The mechanistic importance of the dynein Light Intermediate Chain homologs, LIC1 and LIC2 is unappreciated, especially in the context of mitosis ...
Sagar P Mahale +2 more
doaj +1 more source
Expression and function analysis of mitotic checkpoint genes identifies TTK as a potential therapeutic target for human hepatocellular carcinoma. [PDF]
PLoS ONE, 2014 The mitotic spindle checkpoint (SAC) genes have been considered targets of anticancer therapies. Here, we sought to identify the attractive mitotic spindle checkpoint genes appropriate for human hepatocellular carcinoma (HCC) therapies.
Xiao-Dong Liang +12 more
doaj +1 more source
Long-Range Repulsion Between Chromosomes in Mammalian Oocyte Spindles [PDF]
arXiv, 2022 During eukaryotic cell division, a microtubule-based structure called the spindle exerts forces on chromosomes, thereby organizing and segregating them Extensive work demonstrates that the forces acting parallel to the spindle axis, including those responsible for separating sister chromatids, are generated by microtubule polymerization and ...
arxiv
The equatorial position of the metaphase plate ensures symmetric cell divisions
eLife, 2015 Chromosome alignment in the middle of the bipolar spindle is a hallmark of metazoan cell divisions. When we offset the metaphase plate position by creating an asymmetric centriole distribution on each pole, we find that metaphase plates relocate to the ...
Chia Huei Tan +6 more
doaj +1 more source
Modelling malignant progression with a finite state machine supports a two checkpoint theory of cancer [PDF]
BioDiscovery, 2012 We postulate the two checkpoints theory of cancer, a model of cancer development suggesting that malignant transformation of cells requires loss of function of both the G1 checkpoint and the mitotic spindle checkpoint.
Robert C. Jackson
doaj +3 more sources
Biology Open, 2015 When cells enter mitosis, the anaphase-promoting complex/cyclosome (APC/C) is activated by phosphorylation and binding of Cdc20. The RXXL destruction box (D-box) of cyclin B1 only binds Cdc20 after release of the spindle checkpoint in metaphase ...
Erik Voets, Rob Wolthuis
doaj +1 more source
Fission yeast Wee1 is required for stable kinetochore-microtubule attachment
Open BiologyWee1 is a cell cycle regulator that phosphorylates Cdk1/Cdc2 and inhibits G2/M transition. Loss of Wee1 in fission yeast results in an early onset of mitosis.
Masahiro Takado +3 more
doaj +1 more source