Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes. [PDF]
PLoS ONE, 2007 The spindle checkpoint delays anaphase onset until all chromosomes have attached in a bi-polar manner to the mitotic spindle. Mad and Bub proteins are recruited to unattached kinetochores, and generate diffusible anaphase inhibitors.
Patricia E Rischitor +2 more
doaj +1 more source
Animal cell cytokinesis: the role of dynamic changes in the plasma membrane proteome and lipidome [PDF]
, 2016 In animal cells, cytokinesis is characterised by the formation of the mitotic spindle that signals the assembly of an actomyosin ring between the spindle poles.
Gould, Gwyn W.
core +2 more sources
BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma
FEBS Open Bio, EarlyView.The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru +6 more
wiley +1 more source
Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation [PDF]
, 2012 Haspin phosphorylates histone H3 at threonine-3 (H3T3ph), providing a docking site for the Aurora B complex at centromeres. Aurora B functions to correct improper kinetochore–microtubule attachments and alert the spindle checkpoint to the presence of ...
Daum, John R. +6 more
core +1 more source
Advanced Functional Materials, EarlyView.A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam +8 more
wiley +1 more source
Fission yeast Wee1 is required for stable kinetochore-microtubule attachment
Open BiologyWee1 is a cell cycle regulator that phosphorylates Cdk1/Cdc2 and inhibits G2/M transition. Loss of Wee1 in fission yeast results in an early onset of mitosis.
Masahiro Takado +3 more
doaj +1 more source
The equatorial position of the metaphase plate ensures symmetric cell divisions
eLife, 2015 Chromosome alignment in the middle of the bipolar spindle is a hallmark of metazoan cell divisions. When we offset the metaphase plate position by creating an asymmetric centriole distribution on each pole, we find that metaphase plates relocate to the ...
Chia Huei Tan +6 more
doaj +1 more source
Modelling malignant progression with a finite state machine supports a two checkpoint theory of cancer [PDF]
BioDiscovery, 2012 We postulate the two checkpoints theory of cancer, a model of cancer development suggesting that malignant transformation of cells requires loss of function of both the G1 checkpoint and the mitotic spindle checkpoint.
Robert C. Jackson
doaj +3 more sources
A new survivin tracer tracks, delocalizes and captures endogenous survivin at different subcellular locations and in distinct organelles [PDF]
, 2016 Survivin, the smallest member of the inhibitor of apoptosis protein family, plays a central role during mitosis and exerts a cytoprotective function. Survivin is highly expressed in most cancer types and contributes to multiple facets of carcinogenesis ...
Beghein, Els +5 more
core +2 more sources
Separase prevents genomic instability by controlling replication fork speed [PDF]
, 2016 Proper chromosome segregation is crucial for preserving genomic integrity, and errors in this process cause chromosome mis-segregation, which may contribute to cancer development.
Cucco, Francesco +11 more
core +6 more sources