Building a bridge to a new spindle pole body [PDF]
J Cell Biol, 2015 ![Figure][1] In a wild-type cell, a satellite SPB (S) begins to assemble at the end of a bridge (B) extending from a mother SPB. [Seybold et al][2] describe how three proteins interact to form a structure that initiates the duplication of spindle ...
B. Short
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Anatomy of the fungal microtubule organizing center, the spindle pole body. [PDF]
Curr Opin Struct Biol, 2021 The fungal kingdom is large and diverse, representing extremes of ecology, life cycles and morphology. At a cellular level, the diversity among fungi is particularly apparent at the spindle pole body (SPB). This nuclear envelope embedded structure, which is essential for microtubule nucleation, shows dramatically different morphologies between ...
Jaspersen SL.
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Is cohesin required for spindle-pole-body/centrosome cohesion? [PDF]
Commun Integr Biol, 2012 Centrosomes are microtubule-organizing centers that nucleate spindle microtubules during cell division. In budding yeast, the centrosome, often referred to as the spindle pole body, shares structural components with the centriole, the central core of the
Jin H, Avey M, Yu HG.
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Lessons from yeast: the spindle pole body and the centrosome. [PDF]
Philos Trans R Soc Lond B Biol Sci, 2014 The yeast spindle pole body (SPB) is the functional equivalent of the centrosome. Most SPB components have been identified and their functions partly established. This involved a large variety of techniques which are described here, and the potential use of some of these in the centrosome field is highlighted.
Kilmartin JV.
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Identification of Saccharomyces cerevisiae spindle pole body remodeling factors. [PDF]
PLoS One, 2010 The Saccharomyces cerevisiae centrosome or spindle pole body (SPB) is a dynamic structure that is remodeled in a cell cycle dependent manner. The SPB increases in size late in the cell cycle and during most cell cycle arrests and exchanges components ...
Greenland KB +4 more
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Duplication of the Yeast Spindle Pole Body Once per Cell Cycle. [PDF]
Mol Cell Biol, 2016 The yeast spindle pole body (SPB) is the functional equivalent of the mammalian centrosome. Centrosomes and SPBs duplicate exactly once per cell cycle by mechanisms that use the mother structure as a platform for the assembly of the daughter. The conserved Sfi1 and centrin proteins are essential components of the SPB duplication process.
Rüthnick D, Schiebel E.
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Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha. [PDF]
Elife, 2017 Cytoplasmic microtubules (cMT) control mitotic spindle positioning in many organisms, and are therefore pivotal for successful cell division. Despite its importance, the temporal control of cMT formation remains poorly understood.
Maekawa H +5 more
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A ternary membrane protein complex anchors the spindle pole body in the nuclear envelope in budding yeast. [PDF]
J Biol Chem, 2017 In budding yeast (Saccharomyces cerevisiae) the multilayered spindle pole body (SPB) is embedded in the nuclear envelope (NE) at fusion sites of the inner and outer nuclear membrane.
Kupke T, Malsam J, Schiebel E.
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Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast [PDF]
bioRxiv, 2019 Temperature-sensitive (ts) mutants provide powerful tools, thereby investigating cellular functions of essential genes. We report here a simple procedure to generate ts mutations using error-prone PCR in pcp1 that encodes a spindle pole body (SPB ...
Ngang Heok Tang +5 more
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Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast. [PDF]
Mol Biol Cell, 2014 A previous model suggested doubling of Sfi1 as the first step of SPB assembly. Here it is shown that Sfi1 is gradually recruited to SPBs throughout the cell cycle.
Lee IJ +8 more
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