Disjunction of conjoined twins: Cdk1, Cdh1 and separation of centrosomes
Cell Division, 2006 Accurate transmission of chromosomes from parent to progeny cell requires assembly of a bipolar spindle. Centrosomes (spindle pole body in yeast) are critical for the biogenesis of this complex mitotic apparatus since they confer bipolarity on the ...
Surana Uttam, Crasta Karen
doaj +1 more source
Centriole assembly and the role of Mps1: defensible or dispensable?
Cell Division, 2011 The Mps1 protein kinase is an intriguing and controversial player in centriole assembly. Originally shown to control duplication of the budding yeast spindle pole body, Mps1 is present in eukaryotes from yeast to humans, the nematode C.
Fisk Harold A, Pike Amanda N
doaj +1 more source
A Taz1- and Microtubule-Dependent Regulatory Relationship between Telomere and Centromere Positions in Bouquet Formation Secures Proper Meiotic Divisions. [PDF]
PLoS Genetics, 2016 During meiotic prophase, telomeres cluster, forming the bouquet chromosome arrangement, and facilitate homologous chromosome pairing. In fission yeast, bouquet formation requires switching of telomere and centromere positions.
Kazuhiro Katsumata +8 more
doaj +1 more source
A permeabilized cell model for studying cell division: a comparison of anaphase chromosome movement and cleavage furrow constriction in lysed PtK1 cells. [PDF]
, 1981 After lysis in a Brij 58-polyethylene glycol medium, PtK1 cells are permeable to small molecules, such as erythrosin B, and to proteins, such as rhodamine-labeled FAB, myosin subfragment-1, and tubulin.
CANDE, W. Zacheus +2 more
core +2 more sources
Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body. [PDF]
The Journal of cell biology, 1994 The Saccharomyces cerevisiae genes KAR1 and CDC31 are required for the initial stages of spindle pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KAR1 (Vallen,
S, Biggins, M D, Rose
openaire +2 more sources
Bub3 is a spindle assembly checkpoint protein regulating chromosome segregation during mouse oocyte meiosis. [PDF]
PLoS ONE, 2009 In mitosis, the spindle assembly checkpoint (SAC) prevents anaphase onset until all chromosomes have been attached to the spindle microtubules and aligned correctly at the equatorial metaphase plate.
Mo Li +6 more
doaj +1 more source
Is cohesin required for spindle-pole-body/centrosome cohesion? [PDF]
Commun Integr Biol, 2012 Centrosomes are microtubule-organizing centers that nucleate spindle microtubules during cell division. In budding yeast, the centrosome, often referred to as the spindle pole body, shares structural components with the centriole, the central core of the animal centrosome. The parental centrosome is duplicated when DNA replication takes place.
Jin H, Avey M, Yu HG.
europepmc +4 more sources
Bod1, a novel kinetochore protein required for chromosome biorientation [PDF]
, 2007 We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein
Andersen, Jens S. +7 more
core +4 more sources
Identification of the augmin complex in the filamentous fungus Aspergillus nidulans. [PDF]
PLoS ONE, 2014 Augmin is a protein complex that binds to spindle microtubules (MTs), recruits the potent MT nucleator, γ-tubulin, and thereby promotes the centrosome-independent MT generation within mitotic and meiotic spindles.
Tomoya Edzuka +4 more
doaj +1 more source
Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2 [PDF]
, 2004 Copyright @ The Rockefeller University PressThe guanine nucleotide exchange factor Cdc24, the GTPase Cdc42, and the Cdc42 effectors Cla4 and Ste20, two p21-activated kinases, form a signal transduction cascade that promotes mitotic exit in yeast.
Höfken, T, Schiebel, E
core +2 more sources