Results 61 to 70 of about 10,182 (283)
Molecular Oncology, EarlyView.B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez +17 more
wiley +1 more source
TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes. [PDF]
PLoS Genetics, 2017 Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility.
Müge Akpınar +6 more
doaj +1 more source
Control of 3' splice site selection by the yeast splicing factor Fyv6. [PDF]
ElifePre-mRNA splicing is catalyzed in two steps: 5ʹ splice site (SS) cleavage and exon ligation. A number of proteins transiently associate with spliceosomes to specifically impact these steps (first and second step factors).
Senn KA +5 more
europepmc +2 more sources
Traveling Rocky Roads: The Consequences of Transcription-Blocking DNA Lesions on RNA Polymerase II [PDF]
, 2016 The faithful transcription of eukaryotic genes by RNA polymerase II (RNAP2) is crucial for proper cell function and tissue homeostasis. However, transcription-blocking DNA lesions of both endogenous and environmental origin continuously challenge the ...
Marteijn, Jurgen A., Steurer, Barbara
core +2 more sources
Spliceosome assembly in yeast. [PDF]
Genes & Development, 1987 Precursors to mRNA become substrates for splicing by being assembled into a complex multisubunit structure, the spliceosome. To study the assembly of the yeast spliceosome, intermediate complexes were separated by electrophoresis on nondenaturing polyacrylamide gels. Four splicing-dependent complexes, A1, A2-1, A2-2, and B, were observed.
Soo-Chen Cheng, John Abelson
openaire +3 more sources
Advanced Science, EarlyView.Maternal pregestational diabetes mellitus (PGDM) induced metabolic stress in fetal mice pancreas, alongside significantly decreased insulin secretion, altered cell‐cell communication, and distinct changes in cellular niches. Abstract Maternal pregestational diabetes mellitus (PGDM) can lead to adverse fetal outcomes, including lasting impacts on ...
Yu Deng +3 more
wiley +1 more source
Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. [PDF]
PLoS ONE, 2012 Splicing of primary transcripts is an essential process for the control of gene expression. Specific conserved sequences in premature transcripts are important to recruit the spliceosome machinery.
Patricia P Coltri, Carla C Oliveira
doaj +1 more source
A Phase‐Separated SR Protein Reprograms Host Pre‐mRNA Splicing to Enhance Disease Susceptibility
Advanced Science, EarlyView.This study identifies SR30, a splicing factor, as a negative regulator of tomato immunity. During Phytophthora infestans infection, the elevated SR30 forms nuclear condensates to suppress the alternative splicing (AS) of defense‐related genes in a phase separation manner.
Dong Yan +11 more
wiley +1 more source
Advanced Science, EarlyView.This study utilizes patient‐derived osteosarcoma organoids and CRISPR screening to identify ERCC6 and HNRNPM as key mediators of cisplatin resistance via the PI3K/AKT pathway and BAX alternative splicing. These findings provide novel insights and potential therapeutic targets for overcoming chemoresistance in osteosarcoma.
Ruiling Xu +11 more
wiley +1 more source
Alternative Splicing for Diseases, Cancers, Drugs, and Databases
The Scientific World Journal, 2013 Alternative splicing is a major diversification mechanism in the human transcriptome and proteome. Several diseases, including cancers, have been associated with dysregulation of alternative splicing.
Jen-Yang Tang +6 more
doaj +1 more source