Results 181 to 190 of about 396,812 (264)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

hnRNP F: An emerging orchestrator of RNA processing and gene expression. [PDF]

iScience
Wang Z, Huang H, Li H.
europepmc   +1 more source

Bamboo‐Slip‐Inspired Conformal Reconfigurable Metasurfaces

Advanced Science, EarlyView.
Bamboo‐slip‐inspired conformal reconfigurable metasurface system. The structure is mainly composed of three parts, which are conformal metasurface, pattern reconfigurable antenna, and control components (such as Field Programmable Gate Array (FPGA)). Conformal metasurfaces draw inspiration from the design of ancient Chinese bamboo slips.
Qifan Li, Shaofeng Zhang, Qiang Feng, Dexiao Xia, Yajie Mu, Xiangjin Ma, Jiaqi Han, Haixia Liu, Guangyao Liu, Long Li   +9 more
wiley   +1 more source

CRYSPER and CUREIPES: two new molecular tools enabling analysis of RNA splicing and C-to-U RNA editing-inducible protein expression at single cell resolution. [PDF]

Nucleic Acids Res
Harnau M, Novykova Y, Weicht J, Schweissthal B, Emde L, Brach J, Haake J, Steenpass L, Fricke S, Meier JC.   +9 more
europepmc   +1 more source

WTAP Transcriptional Suppression by KLF9 Drives Osteoclastogenesis via M6A‐Mediated Regulation of CSF1R Signaling in Estrogen‐Deficient Osteoporosis

Advanced Science, EarlyView.
Scheme of the KLF9/WTAP/YTHDF2/m6A/CSF1R regulatory axis in osteoclastogenesis and estrogen‐deficient osteoporosis. WTAP‐mediated m6A modification of Csf1r mRNA governs osteoclastogenesis via a YTHDF2‐mediated pathway. Pathological upregulation of KLF9 drives Wtap transcription, leading to increased m6A deposition on the 3’‐UTR of Csf1r mRNA.
Chen Shen, Xin Liu, Gaoran Ge, Li Xu, Ziyu Zhang, Chang Lv, Hao Xu, Wenhao Li, Yi Qin, Qifeng Sheng, Qihan Wang, Hongxia Li, Jun Zhou, Huilin Yang, Dechun Geng   +14 more
wiley   +1 more source

A Biofabricated Human Acinus‐on‐a‐Chip Unveils Mechanotransductive Drivers of Ventilator‐Induced Lung Injury via Decoupling Volutrauma and Barotrauma

Advanced Science, EarlyView.
This bioinspired acinus‐on‐a‐chip recapitulates VILI pathology, revealing that volutrauma drives P53/NF‐κB pathways while barotrauma triggers mitochondrial‐Wnt dysregulation. A fibrotic transitional cell cluster was identified. Pharmacological interventions targeting these pathways significantly ameliorated injury, establishing a mechanobiological ...
Heng Lu, Liansheng Liu, Zengyong Hu, Shuxian Fang, Ruohan Ren, Sidi Liu, Meng Zhao, Zhiting Luo, Ziyue Zhang, Wenlong Shao, Qian Liu   +10 more
wiley   +1 more source

Functional Validation of a Novel Homozygous <i>TTN</i> Splice-Site Variant Reveals Aberrant Splicing in Hypertrophic Cardiomyopathy. [PDF]

Hum Mutat
Sun X, Wang D, Wang S, Wan R, Jiang Z, Zhang W, Ouyang J, Shen Y, Hong K.   +8 more
europepmc   +1 more source

Overcoming Drug Resistance by Paclitaxel Resistance in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
In the murine triple‐negative breast cancer (TNBC) model, chemotherapy effectively increases tumor neoantigen burden (TNB). Here, the study constructs a liposomal nanovaccine using antigens derived from in vitro chemotherapy‐treated paclitaxel‐resistant TNBC 4T1 cells.
Bo Chen, Yangyi Wang, Tingting Li, Changqing Guo, Zhe Xie, Zhixian Zhang, Xinghua Han, Wei Wang, Jian Wang, Xiaopeng Ma, Yuanzeng Min   +10 more
wiley   +1 more source

Interpretable distillation reveals that deep learning splicing models suffer from pervasive confounders and blind spots. [PDF]

Genome Biol
Liu S, Zhang W, Regev O.
europepmc   +1 more source

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source