Results 31 to 40 of about 3,491 (191)
Naturally Occurring CodY Variants Alter Ligand Binding, DNA Target Affinity, and Virulence in Clostridioides difficile. [PDF]
Mol MicrobiolSingle amino acid substitutions in the global regulator CodY can alter nutrient sensing and virulence regulation in Clostridioides difficile. We show that the CodY(Y146N) and CodY(V58A) variants display altered ligand binding and reduced promoter binding, leading to derepression of toxin regulatory pathways and increased virulence in vivo.
Hasan MK, Roy S, Govind R.
europepmc +2 more sources
Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein [PDF]
Journal of Bacteriology, 1985 The mutation sof-1 suppresses the sporulation defect of mutations in either the spo0B, spo0E, or spo0F stage 0 sporulation genes. Through the use of integrative plasmids carrying the portion of the chromosome including the spo0A locus and flanking regions, the sof-1 mutation was localized near the spo0A locus.
J A, Hoch +3 more
openaire +2 more sources
Soj Antagonizes Spo0A Activation of Transcription in Bacillus subtilis [PDF]
Journal of Bacteriology, 2005 ABSTRACT The ParA family protein Soj appears to negatively regulate sporulation in Bacillus subtilis by inhibiting transcription from promoters that are activated by phosphorylated Spo0A. We tested in vitro Soj inhibition of Spo0A-independent variants of a promoter that Soj inhibited (P spoIIG
Brett N, McLeod, George B, Spiegelman
openaire +2 more sources
Dimer-induced signal progation in Spo0A
Spo0A, the response regulator protein controlling the initiation of sporulation in Bacillus, has two distinct domains, an N-terminal phosphoacceptor (or receiver) domain and a C-terminal DNA-binding (or effector) domain.The phosphoacceptor domain ...
Brannigan JA +7 more
core +4 more sources
mBio, 2010 Bacillus subtilis cells form multicellular biofilm communities in which spatiotemporal regulation of gene expression occurs, leading to differentiation of multiple coexisting cell types.
Claudio Aguilar +4 more
doaj +1 more source
Influence of B. subtilis 3NA mutations in spo0A and abrB on surfactin production in B. subtilis 168
Microbial Cell Factories, 2021 Background Bacillus subtilis is a well-established host for a variety of bioproduction processes, with much interest focused on the production of biosurfactants such as the cyclic lipopeptide surfactin.
Peter Klausmann +6 more
doaj +1 more source
, 2023 The indicated strains were grown for 3 h at 37°C in MDS and an equal amount of cell protein extract for each culture was subjected to Phos-Tag gel for separation of unphosphorylated and phosphorylated Spo0A (Spo0A~P) species.
Francisco A. Uzal (7841351) +5 more
core +1 more source
mBio, 2015 Multicellular biofilm formation and surface motility are bacterial behaviors considered mutually exclusive. However, the basic decision to move over or stay attached to a surface is poorly understood.
Roberto R. Grau +11 more
doaj +1 more source
CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
Scientific Reports, 2021 Bacteria that reside in the gastrointestinal tract of healthy humans are essential for our health, sustenance and well-being. About 50–60% of those bacteria have the ability to produce resilient spores that are important for the life cycle in the gut and
Diogo Martins +6 more
doaj +1 more source
, 2023 The indicated strains were grown in MDS for three (A) or five (B) hours at 37°C. Equal numbers of cells for each strain were lysed and an equal amount of total proteins was subjected to Western blot analysis for Spo0A production using rabbit polyclonal ...
Francisco A. Uzal (7841351) +5 more
core +1 more source