Evidence that spo0A mutations are recessive in spo0A-/spo0A+ merodiploid strains of Bacillus subtilis [PDF]
Journal of Bacteriology, 1978 The spo0A locus contains two types of closely linked mutations that block sporulation at stage 0: spo0A mutations (the most pleiotropic of the stage 0 markers) and spo0C mutations. It was previously thought that spo0A mutations were dominant in merodiploids of Bacillus subtilis, whereas spo0C mutations were recessive. We have shown that spo0A mutations
J, Trowsdale, S M, Chen, J A, Hoch
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Reverse-Engineered Gas-Fermenting Acetogen Strains Recover Enhanced Phenotypes From Autotrophic Adaptive Laboratory Evolution. [PDF]
Microb BiotechnolWe used reverse genetic engineering of the gas‐fermenting model‐acetogen Clostridium autoethanogenum to recover enhanced phenotypes from autotrophic adaptive laboratory evolution. Reverse‐engineered strains were characterised at systems level and bioinformatics suggest that the targeted genes may be involved in overlapping regulatory networks ...
Ingelman H, Shaikh KM, Valgepea K.
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Identification of functional Spo0A residues critical for sporulation in Clostridioides difficile [PDF]
Journal of Molecular Biology, 2022 ABSTRACT Clostridioides difficile is an anaerobic, Gram-positive pathogen that is responsible for C. difficile infection (CDI). To survive in the environment and spread to new hosts, C.
Michael A. DiCandia +5 more
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Complementation of a Clostridium perfringens spo0A Mutant with Wild-Type spo0A from Other Clostridium Species [PDF]
Applied and Environmental Microbiology, 2006 ABSTRACT To evaluate whether C. perfringens can be used as a model organism for studying the sporulation process in other clostridia, C. perfringens spo0A mutant IH101 was complemented with wild-type spo0A from four different Clostridium
I-Hsiu, Huang, Mahfuzur R, Sarker
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A Cell-Penetrating Peptide-Derived Fluorescent Probe for Selective Isolation of Bacterial Spores. [PDF]
Microb BiotechnolOrn8‐K‐FAM selectively labels bacterial spores with minimal vegetative‐cell labeling, enabling fluorescence‐guided isolation of spores from complex environmental microbial communities (created with BioRender.com). ABSTRACT In this study, we identify a peptide discovered through fluorescence‐based screening of a cell‐penetrating peptide (CPP) library ...
Yu J +5 more
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Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis [PDF]
Journal of Bacteriology, 1992 The spoIIG promoter is used by RNA polymerase containing sigma A (E sigma A), the primary form of RNA polymerase found in vegetative cells in Bacillus subtilis. However, the spoIIG promoter is active only after the onset of sporulation. Activation of the spoIIG promoter requires the product of the spo0A gene (Spo0A).
S W, Satola, J M, Baldus, C P, Moran
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A Secreted Factor Coordinates Environmental Quality with Bacillus Development.
PLoS ONE, 2015 Entry into sporulation is governed by the master regulator Spo0A. Spo0A accumulates in its active form, Spo0A-P, as cells enter stationary phase. Prior reports have shown that the acute induction of constitutively active Spo0A during exponential growth ...
Qutaiba O Ababneh +2 more
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Pulsed feedback defers cellular differentiation. [PDF]
PLoS Biology, 2012 Environmental signals induce diverse cellular differentiation programs. In certain systems, cells defer differentiation for extended time periods after the signal appears, proliferating through multiple rounds of cell division before committing to a new ...
Joe H Levine +3 more
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A new mutation inspo0Awith intragenic suppressors in the effector domain [PDF]
FEMS Microbiology Letters, 2000 Spo0A is a two domain response regulator, a key protein in the initiation of sporulation of Bacillus subtilis. This protein controls a number of changes in gene expression that occur during the transition from stationary phase to the onset of sporulation. The phosphorylated form of Spo0A influences the transcription of a specific set of genes.
F, Schmeisser +5 more
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Characterization of the spo0A locus and its deduced product. [PDF]
Proceedings of the National Academy of Sciences, 1985 The highly pleiotropic stage 0 sporulation locus of Bacillus subtilis, spo0A, has been cloned in bacteriophage lambda, subcloned in plasmids, and sequenced. The locus was found to code for a protein of 29,691 Da. Analysis of the in vivo transcripts from this region by nuclease S1 protection experiments located the start and stop of transcription of the
F A, Ferrari +5 more
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