Results 81 to 90 of about 3,491 (191)

Metabolic Origin, Role and Fate of the Denaturant Guanidine

Microbial Biotechnology, Volume 18, Issue 11, November 2025.
The origin of metabolic guanidine is largely a mystery. We suggest it is created when guanine‐containing nucleotides are oxidised by molecular oxygen instead of being broken down into urea as purines normally would. Guanidine may act as a signal to help cells control the level of reactive oxygen species.
Antoine Danchin, Victor de Lorenzo, Pablo Iván Nikel, Conghui You   +3 more
wiley   +1 more source

Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles. [PDF]

PLoS ONE, 2013
Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease.
Yen Kuan Ng, Muhammad Ehsaan, Sheryl Philip, Mark M Collery, Clare Janoir, Anne Collignon, Stephen T Cartman, Nigel P Minton   +7 more
doaj   +1 more source

Balancing Cell Growth and Product Synthesis for Efficient Microbial Cell Factories

Advanced Science, Volume 12, Issue 40, October 27, 2025.
This review presents state‐of‐the‐art metabolic engineering strategies to balance microbial cell growth and product synthesis in biorefineries. It surveys pathway engineering, dynamic genetic circuits, orthogonal control systems, synthetic microbial consortia, and fermentation optimization, alongside integrative modeling approaches.
Linxia Liu, Dongqin Ding, Huiying Wang, Xinyi Ren, Sang Yup Lee, Dawei Zhang   +5 more
wiley   +1 more source

Flexibility and constraint: Evolutionary remodeling of the sporulation initiation pathway in Firmicutes.

PLoS Genetics, 2018
The evolution of signal transduction pathways is constrained by the requirements of signal fidelity, yet flexibility is necessary to allow pathway remodeling in response to environmental challenges.
Philip Davidson, Rory Eutsey, Brendan Redler, N Luisa Hiller, Michael T Laub, Dannie Durand   +5 more
doaj   +1 more source

Comparative Genome Analysis of Three Halobacillus Strains Isolated From Saline Environments Reveal Potential Salt Tolerance and Algicidal Mechanisms

Environmental Microbiology Reports, Volume 17, Issue 3, June 2025.
Following the previous complete genome analysis, this research provides the function of new Halobacillus species. Three strains were identified: Halobacillus shinanisalinarum sp. nov. SSTM10‐2T, Halobacillus salinarum sp. nov. SSBR10‐3T, and Halobacillus amylolyticus sp. nov. SSHM10‐5T.
Saru Gurung, Chang‐Muk Lee, Hang‐Yeon Weon, So‐Ra Han, Tae‐Jin Oh   +4 more
wiley   +1 more source

An A257V Mutation in the Bacillus subtilis Response Regulator Spo0A Prevents Regulated Expression of Promoters with Low-Consensus Binding Sites

, 2009
In Bacillus species, the master regulator of sporulation is Spo0A. Spo0A functions by both activating and repressing transcription initiation from target promoters that contain 0A boxes, the binding sites for Spo0A.
Steve D. Seredick, George B. Spiegelman, S. D. Seredick, B. M. Seredick, Barbara M. Seredick, D. Baker, G. B. Spiegelman, David Baker   +7 more
core   +1 more source

Functional Nucleic Acids as Bacterial Biosensors: A Focus on Clostridioides difficile Infection

Analysis &Sensing, Volume 5, Issue 2, March 2025.
Clostridioides difficile (CD) is a Gram‐positive bacteria causing CD infection (CDI), leading to diarrhea and colon inflammation. Here, we provide a comprehensive review of the use of functional nucleic acids, specifically DNA aptamers and DNAzymes, as novel diagnostic molecular tools for detecting CD, including a discussion on the potential to develop
Kristie Chow, Yingfu Li
wiley   +1 more source

Spo0A-stimulated transcription initiation at the bacillus subtilis spoIIG promother

, 2005
As a last resort to insurmountable environmental stresses, the common soil bacterium Bacillus subtilis undergoes a complicated morphological transformation to produce a metabolically dormant spore.
Seredick, Stephen David
core   +1 more source

A Consecutive Genome Engineering Method Reveals a New Phenotype and Regulation of Glucose and Glycerol Utilization in Clostridium Pasteurianum

Engineering in Life Sciences, Volume 25, Issue 1, January 2025.
ABSTRACT Clostridium pasteurianum is a microorganism for production of 1,3‐propanediol (1,3‐PDO) and butanol, but suffers from lacking genetic tools for metabolic engineering to improve product titers. Furthermore, previous studies of C. pasteurianum have mainly focused on single genomic modification.
Tom Nguyen, Luca W. G. Meleski, Minu P. Belavatta, Sivasubramanian Gurumoorthi, Chijian Zhang, Anna‐Lena Heins, An‐Ping Zeng   +6 more
wiley   +1 more source

Influence of microbiota on the growth and gene expression of Clostridioides difficile in an in vitro coculture model

MicrobiologyOpen, Volume 13, Issue 5, October 2024.
The presence of microbiota did not affect the growth of Clostridioides difficile in this study. However, it did influence the expression of C. difficile genes related to sporulation, germination, and virulence, which are crucial for the transmission of the pathogen. In the presence of microbiota, C.
Elisa Martinez, Noémie Berg, Cristina Rodriguez, Georges Daube, Bernard Taminiau   +4 more
wiley   +1 more source