Results 141 to 150 of about 66,303 (280)
RPA-eGFP can be rapidly replaced from ssDNA by Rad51.
, 2014 (A) Schematic illustrating the predicted outcome for an ssDNA curtain experiment (side view) where RPA-eGFP is replaced by unlabeled Rad51. The loss of fluorescence as RPA-eGFP is displaced by Rad51 also coincides with an increase in the length of the ...
Stephanie C. Gergoudis (518880) +6 more
core +1 more source
ssDNA and ssRNA Promote Phase Condensation of SAMHD1
BiochemistrySAMHD1 (SAM domain and HD domain-containing protein 1) is a deoxynucleoside triphosphate triphosphohydrolase (dNTPase) with functions in viral restriction, R-loop resolution, DNA repair, telomere maintenance, ssRNA homeostasis, and regulation of self-nucleic acids. As a dNTPase, SAMHD1 functions as an allosterically activated tetramer, where binding of
Brandon E. Smith +5 more
openaire +2 more sources
Binding affinity of ssDNA pools of each round of selection.
, 2016 Ten nM of ssDNA pool was incubated with 100 nM of β-BuTx and the ratio of ssDNA bound to β-BuTx was determined by nitrocellulose filter binding assay.
Quanshui Fan (404975) +9 more
core +1 more source
Angewandte Chemie International Edition, EarlyView.Two key binding events that collectively determine the catalytic efficiency of trans‐cleavage: (1) the substrate passing through the gap between the REC and NUC domains, determined by Kpass, and (2) the alignment of the substrate with the catalytic triad of the ribonucleoprotein, determined by Kalign.
Jianyu Hu +3 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner +4 more
wiley +1 more source
BMEMat, EarlyView.LSPR biosensing enables rapid, sensitive, and low‐sample‐volume detection of biomarkers, with broad applications in diagnosing cancer, infectious, and chronic diseases. Advances in nanostructure design, micro/nanofabrication, and microfluidic integration have expanded the potential of LSPR sensors in biomedical diagnostics.
Zhaoxin Geng +4 more
wiley +1 more source
Chemistry – A European Journal, EarlyView.A double‐headed bis‐guanine (G) nucleotide (GG) was evaluated as a noncanonical building block in G‐quadruplexes (G4s). Biophysical and computational analyses reveal that GG can directly participate in G‐tetrads and strongly stabilize G4s in a position‐ and topology‐dependent manner. These findings establish design rules for using GG to engineer highly
Krista Urup +5 more
wiley +1 more source
DNA Nanotechnology Meets Peptide and Protein Self‐Assembly
Chemistry – A European Journal, EarlyView.Combining DNA nanotechnology with peptide and protein assembly provides complementary platforms for the rational engineering of functional biomaterials. This Perspective discusses the emerging field of self‐assembling DNA‐peptide and DNA‐protein hybrid systems that combine the structural precision and programmability of DNA nanotechnology with the ...
Marcel Hanke +4 more
wiley +1 more source
Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing
Exploration, EarlyView.This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang +8 more
wiley +1 more source