Results 161 to 170 of about 2,160,464 (190)
Stable Isotope Probing — Linking Microbial Identity to Function
Nature Reviews Microbiology, 2005 Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate.
Marc G. Dumont, J Colin Murrell
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RNA Stable Isotope Probing (RNA-SIP)
Methods in Molecular Biology, 2019Stable isotope probing is a combined molecular and isotopic technique used to probe the identity and function of uncultivated microorganisms within environmental samples. Employing stable isotopes of common elements such as carbon and nitrogen, RNA-SIP exploits an increase in the buoyant density of RNA caused by the active metabolism and incorporation ...
Benjamin Moreira-Grez +2 more
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Chip-SIP: Stable Isotope Probing Analyzed With rRNA-Targeted Microarrays and NanoSIMS
Methods in Molecular Biology, 2019Chip-SIP is a stable isotope probing (SIP) method for linking microbial identity and function in mixed communities and is capable of analyzing multiple isotopes (13C, 15N, and 18O) simultaneously. This method uses a high-density microarray to separate taxon-specific 16S (or 18S) rRNA genes and a high sensitivity magnetic sector secondary ion mass ...
Peter K. Weber +2 more
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Optimizing Ultracentrifugation Conditions for DNA-Based Stable Isotope Probing (DNA-SIP)
Journal of Microbiological Methods, 2020DNA-SIP (DNA-based stable isotope probing) is increasingly being employed in soil microbial ecology to identify those microbes assimilating the 13C/15N labelled substrate. Isopycnic gradient centrifugation is the primary experimental process for conducting DNA-SIP. However, diverse centrifugal conditions have been used in various recent studies.
Huaiying Yao
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Protein-Based Stable Isotope Probing (Protein-SIP) in Functional Metaproteomics
Mass Spectrometry Reviews, 2012AbstractThe community phenotype as the sum of molecular functions of organisms living in consortia strongly depends on interactions within these communities. Therefore, the analyses of the most significant molecules in terms of the phenotype, the proteins, have to be performed on samples without disrupting the meta‐species environment.
Frank Schmidt +2 more
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Protocol for Performing Protein Stable Isotope Probing (Protein-SIP) Experiments
Springer Protocols, 2016Nico Jehmlich, Martin von Bergen
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Bulletin of Environmental Contamination and Toxicology, 2020
Halogenated compounds are one of the largest groups of environmental-hazardous chemicals. The removal of the halogen atom from the substrate is possible by the catalytic activity of a type of enzyme called dehalogenase. Hydrolytic dehalogenases are suggested to be a good biodegradation catalyst for halogenated compounds with potential bioremediation ...
Hemen Sarma, Sanket J. Joshi
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Halogenated compounds are one of the largest groups of environmental-hazardous chemicals. The removal of the halogen atom from the substrate is possible by the catalytic activity of a type of enzyme called dehalogenase. Hydrolytic dehalogenases are suggested to be a good biodegradation catalyst for halogenated compounds with potential bioremediation ...
Hemen Sarma, Sanket J. Joshi
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Raman Stable Isotope Probing (SIP): A Novel Bio-Analytical Tool
TrAC - Trends in Analytical ChemistrySurya P Singh, Rishikesh Pandey
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Insights into the fate of a 13C labelled phenol pulse for stable isotope probing (SIP) experiments
Journal of Microbiological Methods, 2007Stable isotope probing (SIP) using DNA or RNA as a biomarker has proven to be a useful method for attributing substrate utilisation to specific microbial taxa. In this study we followed the transfer of a (13)C(6)-phenol pulse in an activated sludge micro-reactor to examine the resulting distribution of labelled carbon in the context of SIP. Most of the
Manefield, Mike +5 more
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RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment
2019The RNA-SIP technology allows for linking the structure and function of complex microbial communities, that is, the identification of microbial key players involved in distinct degradation and assimilation processes under in situ conditions. Being dependent on RNA, this technique is particularly suited for environments with high numbers of very active,
Weis, Severin +2 more
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