Results 171 to 180 of about 2,160,464 (190)
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RNA-based stable isotope probing (RNA-SIP) to unravel intestinal host-microbe interactions
Methods, 2018The RNA-SIP technology, introduced into molecular microbial ecology in 2002, is an elegant technique to link the structure and function of complex microbial communities, i.e. to identify microbial key-players involved in distinct degradation and assimilation processes under in-situ conditions.
Egert, Markus +2 more
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2022
ABSTRACT Background Linking the identity of wild microbes with their ecophysiological traits and environmental functions is a key ambition for microbial ecologists. Of many techniques that strive to meet this goal, Stable Isotope Probing—SIP—remains the most comprehensive for ...
Erin E. Nuccio +13 more
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ABSTRACT Background Linking the identity of wild microbes with their ecophysiological traits and environmental functions is a key ambition for microbial ecologists. Of many techniques that strive to meet this goal, Stable Isotope Probing—SIP—remains the most comprehensive for ...
Erin E. Nuccio +13 more
openaire +1 more source
Water Research, 2018
Stable isotope probing (SIP) is a cultivation-independent approach identifying the functional microbes in their natural habitats, possibly linking their identities to functions. DNA-SIP is well-established but suffers from the shift of 12C-DNA into the heavy DNA (13C-DNA) fraction, which significantly reduces the resolution and accuracy. In this study,
Dayi Zhang, Gan Zhang, Chunling Luo
exaly +3 more sources
Stable isotope probing (SIP) is a cultivation-independent approach identifying the functional microbes in their natural habitats, possibly linking their identities to functions. DNA-SIP is well-established but suffers from the shift of 12C-DNA into the heavy DNA (13C-DNA) fraction, which significantly reduces the resolution and accuracy. In this study,
Dayi Zhang, Gan Zhang, Chunling Luo
exaly +3 more sources
Rapid Communications in Mass Spectrometry, 2008
Abstract The identification of metabolically active microbial key players is fundamental for understanding the structure and functions of contaminant‐degrading communities. The metabolic activity can be analysed by feeding the microbial culture with stable‐isotope‐labelled substrates and subsequently tracing their
Nico, Jehmlich +5 more
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Abstract The identification of metabolically active microbial key players is fundamental for understanding the structure and functions of contaminant‐degrading communities. The metabolic activity can be analysed by feeding the microbial culture with stable‐isotope‐labelled substrates and subsequently tracing their
Nico, Jehmlich +5 more
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Science of The Total Environment, 2023
Bioaugmentation (BA) is an effective approach to remove polycyclic aromatic hydrocarbons (PAHs) from contaminated soils, and biochar is frequently used to enhance PAH degradation performance. In this study, phenanthrene (PHE) degradation behavior and active degraders in a petroleum-contaminated soil were investigated and compared between free-cell ...
Tingting, Teng +4 more
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Bioaugmentation (BA) is an effective approach to remove polycyclic aromatic hydrocarbons (PAHs) from contaminated soils, and biochar is frequently used to enhance PAH degradation performance. In this study, phenanthrene (PHE) degradation behavior and active degraders in a petroleum-contaminated soil were investigated and compared between free-cell ...
Tingting, Teng +4 more
openaire +2 more sources
Chemical Engineering Journal, 2018
Abstract The effect of electron acceptors on denitrifying phosphorus (P) removal and its community structures in A2O process treating domestic wastewater was investigated using DNA based stable-isotope probing (DNA-SIP). Stable denitrifying P removal in anoxic zone of A2O process was achieved with an average denitrifying P removal efficiency of 72 ...
Wei Zeng
exaly +2 more sources
Abstract The effect of electron acceptors on denitrifying phosphorus (P) removal and its community structures in A2O process treating domestic wastewater was investigated using DNA based stable-isotope probing (DNA-SIP). Stable denitrifying P removal in anoxic zone of A2O process was achieved with an average denitrifying P removal efficiency of 72 ...
Wei Zeng
exaly +2 more sources
Analytical and Bioanalytical Chemistry, 2011
One of the features of protein-based stable isotope probing is the parallel identification of differentially labeled peptide forms and the accurate calculation of their relative isotope abundances. The level of incorporation is informative of the metabolic activity of the species that synthesized the said protein and peptide.
Martin, Taubert +3 more
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One of the features of protein-based stable isotope probing is the parallel identification of differentially labeled peptide forms and the accurate calculation of their relative isotope abundances. The level of incorporation is informative of the metabolic activity of the species that synthesized the said protein and peptide.
Martin, Taubert +3 more
openaire +2 more sources
Proteomics, 2011
AbstractThe detection of induced proteins after introduction of specific substrates in culture is of high interest for a comparative description of organisms growing under different conditions. In this study, protein‐based stable isotope probing (Protein‐SIP) is used for a fast and reliable detection of newly synthesized proteins in a substrate shift ...
Frank Schmidt +2 more
exaly +3 more sources
AbstractThe detection of induced proteins after introduction of specific substrates in culture is of high interest for a comparative description of organisms growing under different conditions. In this study, protein‐based stable isotope probing (Protein‐SIP) is used for a fast and reliable detection of newly synthesized proteins in a substrate shift ...
Frank Schmidt +2 more
exaly +3 more sources
Environmental Science & Technology, 2022
Abstract Magnetic-nanoparticle-mediated isolation coupled with stable-isotope probing (MMI–SIP) is a cultivation-independent higher-resolution approach for isolating active degraders in their natural habitats. However, it addresses the community level and cannot directly link the microbial identities, phenotypes, and in situ functions
Jibing Li +4 more
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Abstract Magnetic-nanoparticle-mediated isolation coupled with stable-isotope probing (MMI–SIP) is a cultivation-independent higher-resolution approach for isolating active degraders in their natural habitats. However, it addresses the community level and cannot directly link the microbial identities, phenotypes, and in situ functions
Jibing Li +4 more
openaire +2 more sources
Soil Science and Plant Nutrition, 2012
Cyanobacteria play an important role in maintaining soil fertility in rice fields. Some cyanobacterial members can grow heterotrophically under dark conditions, which might be an important trait for the survival of cyanobacteria during the drained period after harvest.
Chol Gyu Lee +4 more
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Cyanobacteria play an important role in maintaining soil fertility in rice fields. Some cyanobacterial members can grow heterotrophically under dark conditions, which might be an important trait for the survival of cyanobacteria during the drained period after harvest.
Chol Gyu Lee +4 more
openaire +1 more source